ArticleJournal of clinical microbiology2003
New methods to assess susceptibilities of Aspergillus isolates to caspofungin.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- A Comprehensive Review on Bioactive Molecules and Advanced Microorganism Management Technologies.Current issues in molecular biology · 2024Review
- Commercial Methods for Antifungal Susceptibility Testing of Saprophytic Molds: Can They Be Used to Detect Resistance?Journal of fungi (Basel, Switzerland) · 2024Review
- Antimicrobial Compounds from Microorganisms.Antibiotics (Basel, Switzerland) · 2022Review
- Multiresistance to Nonazole Fungicides in Aspergillus fumigatus TRAntimicrobial agents and chemotherapy · 2021Article
- Preliminary Characterization of NP339, a Novel Polyarginine Peptide with Broad Antifungal Activity.Antimicrobial agents and chemotherapy · 2021Article
- Responding to the emergence of antifungal drug resistance: perspectives from the bench and the bedside.Future microbiology · 2018Review
- Methods forJournal of pharmaceutical analysis · 2016Review
- Caspofungin Treatment of Aspergillus fumigatus Results in ChsG-Dependent Upregulation of Chitin Synthesis and the Formation of Chitin-Rich Microcolonies.Antimicrobial agents and chemotherapy · 2015Article
- In vitro antifungal activity of micafungin and caspofungin against dermatophytes isolated from China.Mycopathologia · 2013Article
- Epidemiology and antifungal resistance in invasive Aspergillosis according to primary disease: review of the literature.European journal of medical research · 2011Review
- Fungal echinocandin resistance.Fungal genetics and biology : FG & B · 2010Article
- Role for Fks1 in the intrinsic echinocandin resistance of Fusarium solani as evidenced by hybrid expression in Saccharomyces cerevisiae.Antimicrobial agents and chemotherapy · 2009Article
- Effect of caspofungin on metabolite profiles of Aspergillus species determined by nuclear magnetic resonance spectroscopy.Antimicrobial agents and chemotherapy · 2007Article
- Aspergillus infections in transplant recipients.Clinical microbiology reviews · 2005Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Echinocandins are a group of antifungal agents that target 1,3-beta-glucan synthase, causing disruption of mold growth at cells compromising tips and branch points. In part because echinocandins do not induce clear growth inhibition end points using broth dilution techniques, methods to test susceptibility have not yet been standardized. We developed a novel susceptibility assay that measures growth of Aspergillus species on solid agar media that contain serial dilutions of caspofungin (agar dilution). Results of agar dilution testing of multiple isolates were compared to results obtained by broth microdilution (MIC), microscopic evaluation (minimal effective concentration [MEC]), and a new method to measure fungal burden, quantification of secreted hyphal antigen. MICs obtained by the agar dilution method were within 1 dilution of MECs for 85% of the Aspergillus isolates; the highest agreement was observed for isolates of Aspergillus niger (95%), which were particularly susceptible to caspofungin. Agar dilution MICs were also consistent with those obtained by quantifying antigen secretion. MICs obtained by broth microdilution were different than MICs by any other method. Several Aspergillus isolates with decreased susceptibility to caspofungin were identified. Agar dilution is simple and reproducible, and results were consistent with the results of more technically demanding techniques. This method may be appropriate for use in the clinical laboratory.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.