ArticleJournal of clinical microbiology2003
Rapid identification of commonly encountered Candida species directly from blood culture bottles.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- New strategy for rapid diagnosis and characterization of fungal infections: the example of corneal scrapings.PloS one · 2012Trial
- Translation elongation factor 1-alpha gene as a marker for diagnosing of candidal onychomycosis.Current medical mycology · 2020Article
- Validation of a simplex PCR assay enabling reliable identification of clinically relevant Candida species.BMC infectious diseases · 2018Article
- Performance of Gram staining on blood cultures flagged negative by an automated blood culture system.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Molecular and nonmolecular diagnostic methods for invasive fungal infections.Clinical microbiology reviews · 2014Review
- Multi-probe real-time PCR identification of four common Candida species in blood culture broth.Mycopathologia · 2014Article
- Improved identification of yeast species directly from positive blood culture media by combining Sepsityper specimen processing and Microflex analysis with the matrix-assisted laser desorption ionization Biotyper system.Journal of clinical microbiology · 2011Article
- Rapid identification of yeasts from positive blood culture bottles by pyrosequencing.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- High-throughput identification and quantification of Candida species using high resolution derivative melt analysis of panfungal amplicons.The Journal of molecular diagnostics : JMD · 2010Article
- Species-specific identification of a wide range of clinically relevant fungal pathogens by use of Luminex xMAP technology.Journal of clinical microbiology · 2009Article
- Utility of the germ tube test for direct identification of Candida albicans from positive blood culture bottles.Journal of clinical microbiology · 2008Article
- Multiplex tandem PCR: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens.Journal of clinical microbiology · 2008Article
- Differentiation of Candida albicans from non-albicans yeast directly from blood cultures by Gram stain morphology.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2007Article
- Development of novel real-time PCR assays for detection and differentiation of eleven medically important Aspergillus and Candida species in clinical specimens.Journal of clinical microbiology · 2007Article
- Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens.Journal of clinical microbiology · 2007Article
- [Diagnosing fungal infections: trends and new developments].Wiener medizinische Wochenschrift (1946) · 2007Review
- Simultaneous detection and identification of Candida, Aspergillus, and Cryptococcus species by reverse line blot hybridization.Journal of clinical microbiology · 2006Article
- Biology of the pathogenic yeast Candida glabrata.Folia microbiologica · 2006Review
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Rapid identification and enumeration of Saccharomyces cerevisiae cells in wine by real-time PCR.Applied and environmental microbiology · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a rapid-cycle, real-time PCR method for identifying six Candida spp. directly from BACTEC blood culture bottles. Target sequences in the noncoding internal transcribed spacer regions of the rRNA operon were simultaneously amplified and interrogated with fluorescent probes to identify Candida albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, and C. lusitaniae; these account for 88% of the yeast species isolated from positive blood cultures in our laboratory. Any of the first four species can be identified in a single reaction using two fluorescent hybridization probe sets. The antifungal-resistant species C. krusei and C. lusitaniae are detected in a second reaction, also with two probe sets. The assay was validated with DNA extracted from BACTEC blood culture bottles positive for yeasts (n = 62) and was 100% concordant with culture identification based on biochemical and morphological features of C. albicans (n = 22), C. parapsilosis (n = 10), C. tropicalis (n = 1) C. glabrata (n = 22), C. krusei (n = 2), and C. lusitaniae (n = 1). No cross-reactivity was observed in blood culture samples growing yeasts other than the above-mentioned species (n = 4), in those growing bacteria (n = 12), or in the absence of microbial growth. Our assay allows rapid (</=2 h) and specific detection of the most common Candida spp. directly from positive blood cultures and may facilitate delivery of optimal antifungal therapy.
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