ArticleJournal of clinical microbiology2003
Evaluation of a PCR assay for quantitation of Rickettsia rickettsii and closely related spotted fever group rickettsiae.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 99 citations in OpenAlex.
- A real-time PCR assay for detection of Rickettsia rickettsii to aid in vivo infection monitoring.International journal of medical microbiology : IJMM · 2026Article
- Surveillance of tick-borne pathogens present in ticks (Acari: Ixodidae) removed from companion animals in Louisiana, USA.Journal of medical entomology · 2025Article
- Endothelial Mechanistic Target of Rapamycin Activation with Different Strains ofMicroorganisms · 2024Article
- Spotted fever diagnosis using molecular methods.Revista da Sociedade Brasileira de Medicina Tropical · 2024Review
- Tick-Borne Pathogens Screening Using a Multiplex Real-Time Polymerase Chain Reaction-Based Method.Acta parasitologica · 2023Article
- Development and validation of a novel detection method forFrontiers in microbiology · 2023Article
- Procedure for spotted fever group Rickettsia isolation from limited clinical blood specimens.PLoS neglected tropical diseases · 2022Article
- A forty-year review of Rocky Mountain spotted fever cases in California shows clinical and epidemiologic changes.PLoS neglected tropical diseases · 2022Review
- Experimental Rickettsia typhi Infection in Monodelphis domestica: Implications for Opossums as an Amplifying Host in the Suburban Cycle of Murine Typhus.The American journal of tropical medicine and hygiene · 2022Article
- Spotted Fever: An Undercover Cause of Hemophagocytic Lymphohistiocytosis in the Immediate Postpartum.Case reports in infectious diseases · 2022Article
- Replication Kinetics ofMicroorganisms · 2021Article
- A Novel TaqMan Assay for Detection ofJournal of clinical microbiology · 2019Article
- Diagnosis of spotted fever groupEpidemiology and infection · 2019Review
- Article
- Vector competence of Amblyomma americanum (Acari: Ixodidae) for Rickettsia rickettsii.Ticks and tick-borne diseases · 2017Article
- Comparative evaluation of two Rickettsia typhi-specific quantitative real-time PCRs for research and diagnostic purposes.Medical microbiology and immunology · 2017Article
- Article
- The Eco-epidemiology of Pacific Coast Tick Fever in California.PLoS neglected tropical diseases · 2016Article
- Comparative growth of spotted fever group Rickettsia spp. strains in Vero cells.Memorias do Instituto Oswaldo Cruz · 2016Article
- Comparative value of blood and skin samples for diagnosis of spotted fever group rickettsial infection in model animals.Ticks and tick-borne diseases · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
A spotted fever rickettsia quantitative PCR assay (SQ-PCR) was developed for the detection and enumeration of Rickettsia rickettsii and other closely related spotted fever group rickettsiae. The assay is based on fluorescence detection of SYBR Green dye intercalation in a 154-bp fragment of the rOmpA gene during amplification by PCR. As few as 5 copies of the rOmpA gene of R. rickettsii can be detected. SQ-PCR is suitable for quantitation of R. rickettsii and 10 other genotypes of spotted fever group rickettsiae but not for R. akari, R. australis, R. bellii, or typhus group rickettsiae. The sensitivity of SQ-PCR was comparable to that of a plaque assay using centrifugation for inoculation. The SQ-PCR assay was applied successfully to the characterization of rickettsial stock cultures, the replication of rickettsiae in cell culture, the recovery of rickettsial DNA following different methods of extraction, and the quantitation of rickettsial loads in infected animal tissues, clinical samples, and ticks.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.