ArticleBritish journal of pharmacology2004
Correlation between spontaneous electrical, calcium and mechanical activity in detrusor smooth muscle of the guinea-pig bladder.
Article in British journal of pharmacology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 127 citations in OpenAlex.
- Flexible ultrathin organic electrochemical transistor (OECT)-based portable system for monitoring and stimulation of bladder muscle.Flexible and printed electronics · 2026Article
- Cells and ionic conductances contributing to spontaneous activity in bladder and urethral smooth muscle.The Journal of physiology · 2026Review
- PTHrP attenuates spontaneous contractions in detrusor smooth muscle of the rat bladder by activating spontaneous transient outward potassium currents.Pflugers Archiv : European journal of physiology · 2024Article
- Synthesis, Molecular Docking, and Biological Evaluation of Novel Anthranilic Acid Hybrid and Its Diamides as Antispasmodics.International journal of molecular sciences · 2023Article
- Drug-Delivery Silver Nanoparticles: A New Perspective for Phenindione as an Anticoagulant.Biomedicines · 2023Article
- In Silico, In Vitro, and Ex Vivo Biological Activity of Some Novel Mebeverine Precursors.Biomedicines · 2023Article
- Role of PTHrP in attenuating transient pressure rises and associated afferent nerve activity of the rat bladder.Pflugers Archiv : European journal of physiology · 2022Article
- Igniting CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The intracellular CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Urinary bladder smooth muscle ion channels: expression, function, and regulation in health and disease.American journal of physiology. Renal physiology · 2020Review
- A Novel in situ Approach to Studying Detrusor Smooth Muscle Cells in Mice.Scientific reports · 2020Article
- Spontaneous synaptic drive in detrusor smooth muscle: computational investigation and implications for urinary bladder function.Journal of computational neuroscience · 2019Article
- ATP transients accompany spontaneous contractions in isolated guinea-pig detrusor smooth muscle.Experimental physiology · 2019Article
- A computational model of large conductance voltage and calcium activated potassium channels: implications for calcium dynamics and electrophysiology in detrusor smooth muscle cells.Journal of computational neuroscience · 2019Article
- Effect of high-fat diet-induced obesity on the small-conductance CaInternational urology and nephrology · 2019Article
- Regulation of Spontaneous Contractions in Intact Rat Bladder Strips and the Effects of Hydrogen Peroxide.BioMed research international · 2018Article
- A biophysically constrained computational model of the action potential of mouse urinary bladder smooth muscle.PloS one · 2018Article
- Partial bladder outlet obstruction is associated with decreased expression and function of the small-conductance CaInternational urology and nephrology · 2017Article
- Functional coupling of TRPV4 channels and BK channels in regulating spontaneous contractions of the guinea pig urinary bladder.Pflugers Archiv : European journal of physiology · 2016Article
- Purinergic signalling underlies transforming growth factor-β-mediated bladder afferent nerve hyperexcitability.The Journal of physiology · 2016Article
14 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. To investigate the cellular mechanisms underlying spontaneous excitation of smooth muscle of the guinea-pig urinary bladder, isometric tension was measured in muscle bundles while recording the membrane potential from a cell in the bundle with a microeletrode. Changes in the intracellular calcium concentration ([Ca(2+)](i); calcium transients) were recorded in strips loaded with the fluorescent dye, fura-PE3. 2. In 40% of preparations, individual action potentials and contractions, which were abolished by nifedipine (1 microm), were generated. In the remaining preparations, bursting action potentials and contractions were generated. Contractions were again abolished by nifedipine (1 microm), while higher concentrations of nifedipine (10-30 microm) were required to prevent the electrical activity. 3. Carbachol (0.1 microm) increased the frequency of action potentials and corresponding contractions. Apamin (0.1 microm) potentiated bursting activity and enhanced phasic contraction. Charybdotoxin (CTX, 50 nm) induced prolonged action potentials that generated enlarged contractions. In contrast, levcromakalim (0.1 microm) reduced the frequency of action potentials, action potential bursts and the size of the contractions. 4. Forskolin (0.1 microm), 8-bromoguanosin 3', 5' cyclic monophosphate (8Br-cGMP, 0.1 mm) and Y-26763 (10 microm) suppressed contractions without reducing the amplitude of either action potentials or Ca transients. 5. This paper confirms that action potentials and associated calcium transients are fundamental mechanisms in generating spontaneous contractions in smooth muscles of the guinea-pig bladder. However, in parallel with the excitation-contraction coupling, the sensitivity of the contractile proteins for Ca(2+) may play an important role in regulating spontaneous excitation and can be modulated by cyclic nucleotides and Rho kinase.
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