ArticleApplied and environmental microbiology2003
In vitro and in vivo studies of the Yrp1 protease from Yersinia ruckeri and its role in protective immunity against enteric red mouth disease of salmonids.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- Cell wall disruption, membrane damage, and decrease in the expression of Yrp1 virulence factor inIranian journal of microbiology · 2023Article
- Effect of microplastics on Yersinia ruckeri infection in rainbow trout (Oncorhynchus mykiss).Environmental science and pollution research international · 2022Article
- The temperature-dependent expression of type II secretion system controls extracellular product secretion and virulence in mesophilicFrontiers in cellular and infection microbiology · 2022Article
- Review
- Intracellular Bacterial Infections: A Challenge for Developing Cellular Mediated Immunity Vaccines for Farmed Fish.Microorganisms · 2018Review
- Temperature-Dependent Gene Expression inFrontiers in microbiology · 2018Article
- The Infection Process ofFrontiers in cellular and infection microbiology · 2018Review
- Global proteomic profiling of Yersinia ruckeri strains.Veterinary research · 2017Article
- More Than Gliding: Involvement of GldD and GldG in the Virulence ofFrontiers in microbiology · 2017Article
- Yersinia ruckeri, the causative agent of enteric redmouth disease in fish.Veterinary research · 2015Review
- Temperature-dependent expression of virulence genes in fish-pathogenic bacteria.Frontiers in microbiology · 2015Review
- Identification of specific in vivo-induced (ivi) genes in Yersinia ruckeri and analysis of ruckerbactin, a catecholate siderophore iron acquisition system.Applied and environmental microbiology · 2004Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yersinia ruckeri, the etiological agent of the enteric red mouth disease (ERM) of salmonids, produces Yrp1, a serralysin metalloprotease involved in pathogenesis. We describe here the hydrolytic and immunogenic properties of Yrp1. The protease was able to hydrolyze different matrix and muscle proteins as laminin, fibrinogen, gelatine, actin, and myosin but not type II and IV collagens. In addition, the Yrp1 protein, when inactivated by heat and used as an immunogen, was able to elicit a strong protection against the development of ERM. The analysis of different Y. ruckeri strains with (Azo+) or without (Azo-) Yrp1 activity showed that all of them contained the yrp1 operon. By using yrp1::lacZ operon fusions, protease production analysis, and complementation studies, it was possible to show that an Azo- strain was blocked at the transcription level. The transcriptional study of the yrp1 operon under different environmental conditions showed that it was regulated by osmolarity and temperature, without pH influence. Finally, when beta-galactosidase activity was used as a probe in vivo, the progression of the disease in the fish could be visualized, and the tropism of the bacterium and affected organs could be defined. This system opens a vast field of study not only with regard to fish disease progression but also in pathogen interactions, temporal gene expression, carrier stages, antibiotic resistance selection, etc.
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Registered trials
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