ArticleApplied and environmental microbiology2003
Use of a green fluorescent strain for analysis of Xylella fastidiosa colonization of Vitis vinifera.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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84 citing papers in PubMed, 241 citations in OpenAlex.
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- Article
- Review
- Xylem Embolism and Pathogens: Can the Vessel Anatomy of Woody Plants Contribute toPathogens (Basel, Switzerland) · 2023Review
- Structural variation and spatial polysaccharide profiling of intervessel pit membranes in grapevine.Annals of botany · 2022Article
- Anatomical and biochemical studies of Spartium junceum infected by Xylella fastidiosa subsp. multiplex ST 87.Protoplasma · 2022Article
- Evidence for Seed Transmission ofFrontiers in plant science · 2022Article
- Csp1, a Cold Shock Protein Homolog in Xylella fastidiosa Influences Cell Attachment, Pili Formation, and Gene Expression.Microbiology spectrum · 2021Article
- Aphrophoridae Role inPathogens (Basel, Switzerland) · 2021Review
- Susceptibility toAoB PLANTS · 2021Article
- Functionalized microchannels as xylem-mimicking environment: Quantifying X. fastidiosa cell adhesion.Biophysical journal · 2021Article
- Assessment of the Hyperspectral Data Analysis as a Tool to DiagnosePlants (Basel, Switzerland) · 2021Article
- Phenotypic Characterization and Transformation Attempts Reveal Peculiar Traits ofMicroorganisms · 2020Article
- Bacterial Vector-Borne Plant Diseases: Unanswered Questions and Future Directions.Molecular plant · 2020Review
24 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xylella fastidiosa causes Pierce's disease of grapevine as well as several other major agricultural diseases but is a benign endophyte in most host plants. X. fastidiosa colonizes the xylem vessels of host plants and is transmitted by xylem sap-feeding insect vectors. To understand better the pattern of host colonization and its relationship to disease, we engineered X. fastidiosa to express a green fluorescent protein (Gfp) constitutively and performed confocal laser-scanning microscopic analysis of colonization in a susceptible host, Vitis vinifera. In symptomatic leaves, the fraction of vessels colonized by X. fastidiosa was fivefold higher than in nearby asymptomatic leaves. The fraction of vessels completely blocked by X. fastidiosa colonies increased 40-fold in symptomatic leaves and was the feature of colonization most dramatically linked to symptoms. Therefore, the extent of vessel blockage by bacterial colonization is highly likely to be a crucial variable in symptom expression. Intriguingly, a high proportion (>80%) of colonized vessels were not blocked in infected leaves and instead had small colonies or solitary cells, suggesting that vessel blockage is not a colonization strategy employed by the pathogen but, rather, a by-product of endophytic colonization. We present evidence for X. fastidiosa movement through bordered pits to neighboring vessels and propose that vessel-to-vessel movement is a key colonization strategy whose failure results in vessel plugging and disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.