ArticleApplied and environmental microbiology2003
Specific and sensitive detection of Ralstonia solanacearum in soil on the basis of PCR amplification of fliC fragments.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- MAX3 deficiency recruits protective Pseudomonas via modulating the SL-ABA-flavonoid axis to suppress soil-borne pathogen infection.Nature communications · 2026Article
- Bacteria-phage coevolution drives variation in bacterial wilt disease incidence via resistance-virulence trade-offs.Nature microbiology · 2026Article
- Dominance and natural suppression of bacterial plant pathogens across global soils.Nature communications · 2026Article
- Genome-wide identification and functional analysis of the SAMS gene family in peanut reveal the role of Ah6Q1KS5 in resistance to bacterial wilt.BMC plant biology · 2026Article
- Competition between resident rhizosphere bacteria enhances phytopathogen suppression via emergent antagonism.The ISME journal · 2026Article
- Biofertilizer induces soil disease suppression by activating pathogen suppressive protist taxa.NPJ biofilms and microbiomes · 2026Article
- Pan-genome analysis of Ralstonia pseudosolanacearum associated with tobacco bacterial wilt in China.BMC microbiology · 2025Article
- Development of a chemotactic SynCom bioorganic fertilizer for biocontrol of bacterial wilt in tobacco fields.Applied microbiology and biotechnology · 2025Article
- Interactions with native microbial keystone taxa enhance the biocontrol efficiency of Streptomyces.Microbiome · 2025Article
- Stenotrophomonas maltophilia impedes Bacillus biocontrol of tomato wilt disease by degrading its lipopeptide antibiotics.The ISME journal · 2025Article
- Protorhabditis nematodes and pathogen-antagonistic bacteria interactively promote plant health.Microbiome · 2024Article
- Immunomodulating melatonin-decorated silica nanoparticles suppress bacterial wilt (Ralstonia solanacearum) in tomato (Solanum lycopersicum L.) through fine-tuning of oxidative signaling and rhizosphere bacterial community.Journal of nanobiotechnology · 2024Article
- Phosphorus availability influences disease-suppressive soil microbiome through plant-microbe interactions.Microbiome · 2024Article
- Effects of the invasion ofmSphere · 2024Article
- Predatory protists reduce bacteria wilt disease incidence in tomato plants.Nature communications · 2024Article
- Bacterial wilt affects the structure and assembly of microbial communities along the soil-root continuum.Environmental microbiome · 2024Article
- Tomato growth stage modulates bacterial communities across different soil aggregate sizes and disease levels.ISME communications · 2023Article
- Tapping the rhizosphere metabolites for the prebiotic control of soil-borne bacterial wilt disease.Nature communications · 2023Article
- Loop-mediated isothermal amplification (LAMP)/Cas12a assay for detection ofFrontiers in bioengineering and biotechnology · 2023Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ralstonia solanacearum is the causative agent of bacterial wilt in many important crops. A specific and sensitive PCR detection method that uses primers targeting the gene coding for the flagella subunit, fliC, was established. Based on the first fliC gene sequence of R. solanacearum strain K60 available at GenBank, the Ral_fliC PCR primer system was designed; this system yielded a single 724-bp product with the DNAs of all of the R. solanacearum strains tested. However, R. pickettii and four environmental Ralstonia isolates also yielded amplicons. The Ral_fliC PCR products obtained with 12 strains (R. solanacearum, R. pickettii, and environmental isolates) were sequenced. By sequence alignment, Rsol_fliC primers specific for R. solanacearum were designed. With this primer system, a specific 400-bp PCR product was obtained from all 82 strains of R. solanacearum tested. Six strains of R. pickettii and several closely related environmental isolates yielded no PCR product; however, a product was obtained with one Pseudomonas syzygii strain. A GC-clamped 400-bp fliC product could be separated in denaturing gradient gels and allowed us to distinguish P. syzygii from R. solanacearum. The Rsol_fliC PCR system was applied to detect R. solanacearum in soil. PCR amplification, followed by Southern blot hybridization, allowed us to detect about one target DNA molecule per PCR, which is equivalent to 10(3) CFU g of bulk soil(-1). The system was applied to survey soils from different geographic origins for the presence of R. solanacearum.
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