ArticleApplied and environmental microbiology2003
Improvement of foreign-protein production in Aspergillus niger var. awamori by constitutive induction of the unfolded-protein response.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 113 citations in OpenAlex.
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- Stepwise genetic modification for efficient expression of heterologous proteins in Aspergillus nidulans.Applied microbiology and biotechnology · 2023Article
- Exploration of the Strategy for Improving the Expression of Heterologous Sweet Protein Monellin inJournal of fungi (Basel, Switzerland) · 2023Article
- Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability.Materials today. Bio · 2023Article
- Study of protease activity from Aspergillus awamori INCQS2B.361U2/1 extracellular fraction and modification of culture medium composition to isolate a novel aspartic protease.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Characterization and modulation of endoplasmic reticulum stress response target genes in Kluyveromyces marxianus to improve secretory expressions of heterologous proteins.Biotechnology for biofuels · 2021Article
- Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris.Microbial cell factories · 2021Article
- Protein expression and secretion by filamentous fungi.Journal of biosciences · 2021Review
- A Genomic Catalog of Stress Response Genes in Anaerobic Fungi for Applications in Bioproduction.Frontiers in fungal biology · 2021Article
- Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion.Frontiers in bioengineering and biotechnology · 2020Review
- Mining Data From Plasma Cell Differentiation Identified Novel Genes for Engineering of a Yeast Antibody Factory.Frontiers in bioengineering and biotechnology · 2020Article
- Harnessing the knowledge of protein secretion for enhanced protein production in filamentous fungi.World journal of microbiology & biotechnology · 2019Review
- Identification of an Exceptionally Long Intron in themSphere · 2018Article
- Protein hyperproduction in fungi by design.Applied microbiology and biotechnology · 2018Review
- Altered secretion patterns and cell wall organization caused by loss of PodB function in the filamentous fungus Aspergillus nidulans.Scientific reports · 2018Article
- Protein profile in Aspergillus nidulans recombinant strains overproducing heterologous enzymes.Microbial biotechnology · 2018Article
- Conditional Expression of the Small GTPase ArfA Impacts Secretion, Morphology, Growth, and Actin Ring Position inFrontiers in microbiology · 2018Article
- Regulators of plant biomass degradation in ascomycetous fungi.Biotechnology for biofuels · 2017Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unfolded-protein response (UPR) denotes the upregulation of endoplasmic reticulum (ER)-resident chaperone and foldase genes and numerous other genes involved in secretory functions during the accumulation of unfolded proteins into the ER. Overexpression of individual foldases and chaperones has been used in attempts to improve protein production in different production systems. We describe here a novel strategy to improve foreign-protein production. We show that the constitutive induction of the UPR pathway in Aspergillus niger var. awamori can be achieved by expressing the activated form of the transcription factor hacA. This induction enhances the production of Trametes versicolor laccase by up to sevenfold and of bovine preprochymosin by up to 2.8-fold in this biotechnically important fungus. The regulatory range of UPR was studied by analyzing the mRNA levels of novel A. niger var. awamori genes involved in different secretory functions. This revealed both similarities and differences to corresponding studies in Saccharomyces cerevisiae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.