ArticleProceedings of the National Academy of Sciences of the United States of America2003
Identification of 26RFa, a hypothalamic neuropeptide of the RFamide peptide family with orexigenic activity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 198 citations in OpenAlex.
- Single-variant genome-wide association study and regional heritability mapping of protein efficiency and performance traits in Large White pigs.Genetics, selection, evolution : GSE · 2025Article
- A single injection of neuropeptide QRFP in the lateral hypothalamus decreased food intake.Journal of psychopharmacology (Oxford, England) · 2025Article
- The Art of Chilling Out: How Neurons Regulate Torpor.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- QRFP43 modulates the activity of the hypothalamic-pituitary-thyroid axis in female sheep.Scientific reports · 2025Article
- Photochemically-enabled, post-translational production of C-terminal amides.Nature communications · 2024Article
- Molecular mechanism of prolactin-releasing peptide recognition and signaling via its G protein-coupled receptor.Cell discovery · 2024Article
- Review
- Structure and dynamics of the pyroglutamylated RF-amide peptide QRFP receptor GPR103.Nature communications · 2024Article
- Structural basis for recognition of 26RFa by the pyroglutamylated RFamide peptide receptor.Cell discovery · 2024Article
- Possible roles of neuropeptide/transmitter and autoantibody modulation in emotional problems and aggression.Frontiers in psychiatry · 2024Review
- The hypothalamic neuropeptide, QRFP, regulates high fat diet intake in female Long-Evans rats following ovariectomy.Peptides · 2023Article
- Blood Levels of Neuropeptide 26RFa in Relation to Anxiety and Aggressive Behavior in Humans-An Exploratory Study.Brain sciences · 2023Article
- The 26RFa (QRFP)/GPR103 neuropeptidergic system in mice relays insulin signalling into the brain to regulate glucose homeostasis.Diabetologia · 2022Article
- The hypothalamic RFamide, QRFP, increases feeding and locomotor activity: The role of Gpr103 and orexin receptors.PloS one · 2022Article
- Point-Substitution of Phenylalanine Residues of 26RFa Neuropeptide: A Structure-Activity Relationship Study.Molecules (Basel, Switzerland) · 2021Article
- GIP_HUMAN[22-51] is a new proatherogenic peptide identified by native plasma peptidomics.Scientific reports · 2021Article
- RF-amide related peptide-3 (RFRP-3): a novel neuroendocrine regulator of energy homeostasis, metabolism, and reproduction.Molecular biology reports · 2021Review
- Effects of Caffeine on Brown Adipose Tissue Thermogenesis and Metabolic Homeostasis: A Review.Frontiers in neuroscience · 2021Review
- New Peptides as Potential Players in the Crosstalk Between the Brain and Obesity, Metabolic and Cardiovascular Diseases.Frontiers in physiology · 2021Review
- Article
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A neuropeptide was isolated from a frog brain extract by HPLC purification and characterized by mass spectrometry. This 26-aa neuropeptide, which belongs to the RFamide peptide family, was designated 26RFa, and its primary structure was established as VGTALGSLAEELNGYNRKKGGFSFRF-NH2. Research in databases revealed the presence of sequences homologous to frog 26RFa in the human genome and in rat ESTs. On the basis of this sequence information, the cDNAs encoding the human and rat 26RFa precursors were cloned. The two preproteins show a similar organization, with the 26RFa sequence located in the C-terminal region of the precursor. Human preprotein (prepro)-26RFa encodes an additional putative RFamide peptide that is not found in the rat precursor. The primary structures of human, rat, and frog 26RFa exhibit approximately 80% identity, and the C-terminal octapeptide has been fully conserved from amphibians to mammals. In situ hybridization histochemistry revealed that, in the rat brain, the 26RFa gene is exclusively expressed in the ventromedial hypothalamic nucleus and in the lateral hypothalamic area. 26RFa induced a dose-dependent stimulation in cAMP production by rat pituitary cells in vitro and markedly increased food intake in mice. The conservation of the primary structure of 26RFa during vertebrate evolution, the discrete localization of the mRNA encoding its precursor in hypothalamic nuclei involved in the control of feeding behavior, and the observation that 26RFa possesses orexigenic properties indicate that this neuropeptide may play important biological functions.
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