ArticleMolecular biology of the cell2004
The EF-hand Ca2+-binding protein p22 plays a role in microtubule and endoplasmic reticulum organization and dynamics with distinct Ca2+-binding requirements.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Conformational regulation and target-myristoyl switch of calcineurin B homologous protein 3.eLife · 2023Article
- Structural Diversity within the Endoplasmic Reticulum-From the Microscale to the Nanoscale.Cold Spring Harbor perspectives in biology · 2023Review
- CHP1 reduction ameliorates spinal muscular atrophy pathology by restoring calcineurin activity and endocytosis.Brain : a journal of neurology · 2018Article
- Untangling the web: mechanisms underlying ER network formation.Biochimica et biophysica acta · 2013Review
- ER structure and function.Current opinion in cell biology · 2013Review
- Calcineurin homologous protein: a multifunctional Ca2+-binding protein family.American journal of physiology. Renal physiology · 2012Review
- A calcineurin homologous protein is required for sodium-proton exchange events in the C. elegans intestine.American journal of physiology. Cell physiology · 2011Article
- Nuclear-localized calcineurin homologous protein CHP1 interacts with upstream binding factor and inhibits ribosomal RNA synthesis.The Journal of biological chemistry · 2010Article
- Further assembly required: construction and dynamics of the endoplasmic reticulum network.EMBO reports · 2010Review
- Localization and socialization: experimental insights into the functional architecture of IP3 receptors.Chaos (Woodbury, N.Y.) · 2009Review
- The calcineurin homologous protein-1 increases Na(+)/H(+) -exchanger 3 trafficking via ezrin phosphorylation.Journal of the American Society of Nephrology : JASN · 2009Article
- p31 deficiency influences endoplasmic reticulum tubular morphology and cell survival.Molecular and cellular biology · 2009Article
- FFAT rescues VAPA-mediated inhibition of ER-to-Golgi transport and VAPB-mediated ER aggregation.Journal of cell science · 2008Article
- Calcineurin B homologous protein 3 promotes the biosynthetic maturation, cell surface stability, and optimal transport of the Na+/H+ exchanger NHE1 isoform.The Journal of biological chemistry · 2008Article
- Genetic dissociation of ethanol sensitivity and memory formation in Drosophila melanogaster.Genetics · 2008Article
- Endoplasmic reticulum remains continuous and undergoes sheet-to-tubule transformation during cell division in mammalian cells.The Journal of cell biology · 2007Article
- p180 is involved in the interaction between the endoplasmic reticulum and microtubules through a novel microtubule-binding and bundling domain.Molecular biology of the cell · 2007Article
- Receptor complexes cotransported via polarized endocytic pathways form clusters with distinct organizations.Molecular biology of the cell · 2007Article
- Roles and origins of leukocyte lipid bodies: proteomic and ultrastructural studies.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2007Article
- Endoplasmic reticulum architecture: structures in flux.Current opinion in cell biology · 2006Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have reported that p22, an N-myristoylated EF-hand Ca(2+)-binding protein, associates with microtubules and plays a role in membrane trafficking. Here, we show that p22 also associates with membranes of the early secretory pathway membranes, in particular endoplasmic reticulum (ER). On binding of Ca(2+), p22's ability to associate with membranes increases in an N-myristoylation-dependent manner, which is suggestive of a nonclassical Ca(2+)-myristoyl switch mechanism. To address the intracellular functions of p22, a digitonin-based "bulk microinjection" assay was developed to load cells with anti-p22, wild-type, or mutant p22 proteins. Antibodies against a p22 peptide induce microtubule depolymerization and ER fragmentation; this antibody-mediated effect is overcome by preincubation with the respective p22 peptide. In contrast, N-myristoylated p22 induces the formation of microtubule bundles, the accumulation of ER structures along the bundles as well as an increase in ER network formation. An N-myristoylated Ca(2+)-binding p22 mutant, which is unable to undergo Ca(2+)-mediated conformational changes, induces microtubule bundling and accumulation of ER structures along the bundles but does not increase ER network formation. Together, these data strongly suggest that p22 modulates the organization and dynamics of microtubule cytoskeleton in a Ca(2+)-independent manner and affects ER network assembly in a Ca(2+)-dependent manner.
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