ArticleMolecular biology of the cell2004
Translocation of FGF-1 and FGF-2 across vesicular membranes occurs during G1-phase by a common mechanism.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 55 citations in OpenAlex.
- Impact of FGF1 on human periodontal ligament fibroblast growth, osteogenic differentiation and inflammatory reaction in vitro.Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2022Article
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- Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells.The Journal of cell biology · 2018Article
- Cytotoxic Conjugates of Fibroblast Growth Factor 2 (FGF2) with Monomethyl Auristatin E for Effective Killing of Cells Expressing FGF Receptors.ACS omega · 2017Article
- Identification of new FGF1 binding partners-Implications for its intracellular function.IUBMB life · 2016Article
- Internal ribosome entry site-based vectors for combined gene therapy.World journal of experimental medicine · 2015Review
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- Cell penetration peptides for enhanced entry of αB-crystallin into lens cells.Investigative ophthalmology & visual science · 2013Article
- Steroid-like signalling by interferons: making sense of specific gene activation by cytokines.The Biochemical journal · 2012Review
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- Increased protein stability of FGF1 can compensate for its reduced affinity for heparin.The Journal of biological chemistry · 2009Article
- Fibroblast growth factor-2 regulates the stability of nuclear bodies.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Article
- Phosphorylation of fibroblast growth factor (FGF) receptor 1 at Ser777 by p38 mitogen-activated protein kinase regulates translocation of exogenous FGF1 to the cytosol and nucleus.Molecular and cellular biology · 2008Article
- Article
- Construction and characterization of a thrombin-resistant designer FGF-based collagen binding domain angiogen.Biomaterials · 2008Article
- Bacterial ligands generated in a phagosome are targets of the cytosolic innate immune system.PLoS pathogens · 2007Article
- Nuclear trafficking of secreted factors and cell-surface receptors: new pathways to regulate cell proliferation and differentiation, and involvement in cancers.Cell communication and signaling : CCS · 2006Article
- Fibroblast growth factor 2 induced proliferation in osteoblasts and bone marrow stromal cells: a whole cell model.Biophysical journal · 2006Article
- Phosphorylation-regulated nucleocytoplasmic trafficking of internalized fibroblast growth factor-1.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The entry of exogenous fibroblast growth factor 2 (FGF-2) to the cytosolic/nuclear compartment was studied and compared with the translocation mechanism used by FGF-1. To differentiate between external and endogenous growth factor, we used FGF-2 modified to contain a farnesylation signal, a CaaX-box. Because farnesylation occurs only in the cytosol and nucleoplasm, farnesylation of exogenous FGF-2-CaaX was taken as evidence that the growth factor had translocated across cellular membranes. We found that FGF-2 translocation occurred in endothelial cells and fibroblasts, which express FGF receptors, and that the efficiency of translocation was increased in the presence of heparin. Concomitantly with translocation, the 18-kDa FGF-2 was N-terminally cleaved to yield a 16-kDa form. Translocation of FGF-2 required PI3-kinase activity but not transport through the Golgi apparatus. Inhibition of endosomal acidification did not prevent translocation, whereas dissipation of the vesicular membrane potential completely blocked it. The data indicate that translocation occurs from intracellular vesicles containing proton pumps and that an electrical potential across the vesicle membrane is required. Translocation of both FGF-1 and FGF-2 occurred during most of G(1) but decreased shortly before the G(1)-->S transition. A common mechanism for FGF-1 and FGF-2 translocation into cells is postulated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.