ArticleThe EMBO journal2003
Visualizing telomere dynamics in living mammalian cells using PNA probes.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
91 citing papers in PubMed, 220 citations in OpenAlex.
- Centromeres and telomeres as rheological probes of the human cell nucleus.Biophysical journal · 2026Article
- Increased Telomere Mobility in Progeria is Restored by Isoprenylcysteine Carboxyl Methyltransferase Inhibition.bioRxiv : the preprint server for biology · 2026Article
- Article
- Tracing the Chromatin: From 3C to Live-Cell Imaging.Chemical & biomedical imaging · 2024Review
- Non-random spatial organization of telomeres varies during the cell cycle and requires LAP2 and BAF.iScience · 2024Article
- Detection of Telomere Transfer at Immunological Synapse.Methods in molecular biology (Clifton, N.J.) · 2023Article
- XPF activates break-induced telomere synthesis.Nature communications · 2022Article
- An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory.Nature cell biology · 2022Article
- Shelterin Components Modulate Nucleic Acids Condensation and Phase Separation in the Context of Telomeric DNA.Journal of molecular biology · 2022Article
- Probing local chromatin dynamics by tracking telomeres.Biophysical journal · 2022Article
- Ultrastructure and nuclear architecture of telomeric chromatin revealed by correlative light and electron microscopy.Nucleic acids research · 2022Article
- Fast and parallel nanoscale three-dimensional tracking of heterogeneous mammalian chromatin dynamics.Molecular biology of the cell · 2022Article
- Review
- Compartmentalization of telomeres through DNA-scaffolded phase separation.Developmental cell · 2022Article
- Scaling Relationship in Chromatin as a Polymer.Results and problems in cell differentiation · 2022Article
- Live-cell imaging probes to track chromatin modification dynamics.Microscopy (Oxford, England) · 2021Article
- CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently.Journal of biological engineering · 2021Article
- Bead Loading Proteins and Nucleic Acids into Adherent Human Cells.Journal of visualized experiments : JoVE · 2021Article
- Nuclear envelope tethering inhibits the formation of ALT-associated PML bodies in ALT cells.Aging · 2021Article
- Small molecule inhibitor of OGG1 blocks oxidative DNA damage repair at telomeres and potentiates methotrexate anticancer effects.Scientific reports · 2021Article
31 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome ends are protected from degradation by the presence of the highly repetitive hexanucleotide sequence of TTAGGG and associated proteins. These so-called telomeric complexes are suggested to play an important role in establishing a functional nuclear chromatin organization. Using peptide nucleic acid (PNA) probes, we studied the dynamic behavior of telomeric DNA repeats in living human osteosarcoma U2OS cells. A fluorescent cy3-labeled PNA probe was introduced in living cells by glass bead loading and was shown to specifically associate with telomeric DNA shortly afterwards. Telomere dynamics were imaged for several hours using digital fluorescence microscopy. While the majority of telomeres revealed constrained diffusive movement, individual telomeres in a human cell nucleus showed significant directional movements. Also, a subfraction of telomeres were shown to associate and dissociate, suggesting that in vivo telomere clusters are not stable but dynamic structures. Furthermore, telomeres were shown to associate with promyelocytic leukemia (PML) bodies in a dynamic manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.