ArticleThe EMBO journal2003
Nsl1p is essential for the establishment of bipolarity and the localization of the Dam-Duo complex.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- A TOGL domain specifically targets yeast CLASP to kinetochores to stabilize kinetochore microtubules.The Journal of cell biology · 2014Article
- Detection of protein complexes from affinity purification/mass spectrometry data.BMC systems biology · 2012Article
- Diverse protein kinase interactions identified by protein microarrays reveal novel connections between cellular processes.Genes & development · 2011Article
- CaMtw1, a member of the evolutionarily conserved Mis12 kinetochore protein family, is required for efficient inner kinetochore assembly in the pathogenic yeast Candida albicans.Molecular microbiology · 2011Article
- Molecular architecture and assembly of the yeast kinetochore MIND complex.The Journal of cell biology · 2010Article
- Roles for the conserved spc105p/kre28p complex in kinetochore-microtubule binding and the spindle assembly checkpoint.PloS one · 2009Article
- Function and assembly of DNA looping, clustering, and microtubule attachment complexes within a eukaryotic kinetochore.Molecular biology of the cell · 2009Article
- Structural and functional dissection of Mif2p, a conserved DNA-binding kinetochore protein.Molecular biology of the cell · 2008Article
- Kinetochore structure and spindle assembly checkpoint signaling in the budding yeast, Saccharomyces cerevisiae.Frontiers in bioscience : a journal and virtual library · 2008Review
- Article
- The human kinetochore proteins Nnf1R and Mcm21R are required for accurate chromosome segregation.The EMBO journal · 2006Article
- The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation.The Journal of cell biology · 2006Article
- Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins.Genome biology · 2006Article
- Kinetochore-spindle microtubule interactions during mitosis.Current opinion in cell biology · 2005Review
- Genetic analysis of the kinetochore DASH complex reveals an antagonistic relationship with the ras/protein kinase A pathway and a novel subunit required for Ask1 association.Molecular and cellular biology · 2005Article
- A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension.Genes & development · 2004Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We identified a physical complex consisting of Mtw1p, an established kinetochore protein, with Nnf1p, Nsl1p and Dsn1p and have demonstrated that Nnf1p, Nsl1p and Dsn1p localize to the Saccharomyces cerevisiae kinetochore. When challenged prior to metaphase, the temperature-sensitive mutants nsl1-16 and nsl1-42 as well as Nsl1p-depleted cells failed to establish a bipolar spindle-kinetochore interaction and executed monopolar segregation of sister chromatids. In contrast, an nsl1-16 defect could not be evoked after the establishment of bipolarity. The observed phenotype is characteristic of that of mutants with defects in the protein kinase Ipl1p or components of the Dam-Duo kinetochore complex. However nsl1 mutants did not exhibit a defect in microtubule-kinetochore untethering as the ipl1-321 mutant does. Instead, they exhibited a severe defect in the kinetochore localization of the Dam-Duo complex suggesting this to be the cause for the failure of nsl1 cells to establish bipolarity. Moreover the analysis of Nsl1p-depleted cells indicated that Nsl1p is required for the spindle checkpoint and kinetochore integrity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.