ArticleMolecular genetics and genomics : MGG2004
Two-hybrid search for proteins that interact with Sad1 and Kms1, two membrane-bound components of the spindle pole body in fission yeast.
Article in Molecular genetics and genomics : MGG, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed, 103 citations in OpenAlex.
- Homozygous missense mutation in CCDC155 disrupts the transmembrane distribution of CCDC155 and SUN1, resulting in non-obstructive azoospermia and premature ovarian insufficiency in humans.Human genetics · 2022Article
- Determinants of RPA megafoci localization to the nuclear periphery in response to replication stress.G3 (Bethesda, Md.) · 2022Article
- Quantitative analysis of nuclear pore complex organization inLife science alliance · 2022Article
- Redistribution of centrosomal proteins by centromeres and Polo kinase controls partial nuclear envelope breakdown in fission yeast.Molecular biology of the cell · 2021Article
- High-Throughput Identification of Nuclear Envelope Protein Interactions inG3 (Bethesda, Md.) · 2020Article
- The mechanisms and significance of the positional control of centromeres and telomeres in plants.Journal of plant research · 2020Review
- The TERB1-TERB2-MAJIN complex of mouse meiotic telomeres dates back to the common ancestor of metazoans.BMC evolutionary biology · 2020Article
- Review
- Nuclear Mechanics in the Fission Yeast.Cells · 2019Review
- Review
- Position matters: multiple functions of LINC-dependent chromosome positioning during meiosis.Current genetics · 2017Article
- Nuclear Envelope-Associated Chromosome Dynamics during Meiotic Prophase I.Frontiers in cell and developmental biology · 2017Review
- CSL protein regulates transcription of genes required to prevent catastrophic mitosis in fission yeast.Cell cycle (Georgetown, Tex.) · 2016Article
- Chromosome domain architecture and dynamic organization of the fission yeast genome.FEBS letters · 2015Review
- Making the LINC: SUN and KASH protein interactions.Biological chemistry · 2015Review
- Structural and functional adaptations of the mammalian nuclear envelope to meet the meiotic requirements.Nucleus (Austin, Tex.) · 2015Review
- The KASH protein Kms2 coordinates mitotic remodeling of the spindle pole body.Journal of cell science · 2014Article
- Analysis of meiosis in SUN1 deficient mice reveals a distinct role of SUN2 in mammalian meiotic LINC complex formation and function.PLoS genetics · 2014Article
- Nuclear forces and cell mechanosensing.Progress in molecular biology and translational science · 2014Review
- Chromosome segregation in plant meiosis.Frontiers in plant science · 2014Review
2 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In interphase cells of fission yeast, the spindle pole body (SPB) is thought to be connected with chromosomal centromeres by an as yet unknown mechanism that spans the nuclear membrane. To elucidate this mechanism, we performed two-hybrid screens for proteins that interact with Kms1 and Sad1, which are constitutive membrane-bound components of the SPB that interact with each other. Seven and 26 genes were identified whose products potentially interact with Kms1 and Sad1, respectively. With the exception of Dlc1 (a homolog of the 14-kDa dynein light chain), all of the Kms1 interactors also interacted with Sad1. Among the genes identified were the previously known genes rhp9+ / crb2+, cut6+, ags1+ / mok1+, gst3+, kms2+, and sid4+. The products of kms2+ and sid4+ localize to the SPB. The novel genes were characterized by constructing disruption mutations and by localization of the gene products. Two of them, putative homologues of budding yeast UFE1 (which encodes a t-SNARE) and SFH1 (an essential component of a chromatin-remodeling complex), were essential for viability. Two further genes, which were only conditionally essential, genetically interact with sad1+. One of these was named sif1+ (for Sad1-interacting factor) and is required for proper septum formation at high temperature. Cells in which this gene was overexpressed displayed a wee -like phenotype. The product of the other gene, apm1+, is very similar to the medium chain of an adaptor protein complex in clathrin-coated vesicles. Apm1 appears to be required for SPB separation and spindle formation, and tended to accumulate at the SPB when it was overproduced. It was functionally distinct from its homologues Apm2 and Apm4. Other novel genes identified in this study included one for a nucleoporin and genes encoding novel membrane-bound proteins that were genetically related to Sad1. We found that none of the newly identified genes tested were necessary for centromere/telomere clustering.
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