ArticleNucleic acids research2003
Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Identification of an enzyme with strong single-stranded DNA ligation activity and its application for sequencing.Nucleic acids research · 2025Article
- Ampullaviruses: From Extreme Environments to Biotechnological Innovation.Current pharmaceutical biotechnology · 2025Review
- Functional biology and biotechnology of thermophilic viruses.Essays in biochemistry · 2023Review
- Potential Applications of Thermophilic Bacteriophages in One Health.International journal of molecular sciences · 2023Review
- Quantitative analysis of miRNAs using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER)-qPCR.Molecular therapy. Nucleic acids · 2023Article
- Applications of phage-derived RNA-based technologies in synthetic biology.Synthetic and systems biotechnology · 2020Review
- Microbial Diversity and Phage-Host Interactions in the Georgian Coastal Area of the Black Sea Revealed by Whole Genome Metagenomic Sequencing.Marine drugs · 2020Article
- Highly efficient single-stranded DNA ligation technique improves low-input whole-genome bisulfite sequencing by post-bisulfite adaptor tagging.Nucleic acids research · 2019Article
- Isolation, growth and genome of the Rhodothermus RM378 thermophilic bacteriophage.Extremophiles : life under extreme conditions · 2014Article
- Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase - engineering a thermostable ATP independent enzyme.BMC molecular biology · 2012Article
- Targeted detection of in vivo endogenous DNA base damage reveals preferential base excision repair in the transcribed strand.Nucleic acids research · 2012Article
- Genomic and proteomic characterization of the broad-host-range Salmonella phage PVP-SE1: creation of a new phage genus.Journal of virology · 2011Article
- RNA-ligase-dependent biases in miRNA representation in deep-sequenced small RNA cDNA libraries.RNA (New York, N.Y.) · 2011Article
- miR-ID: a novel, circularization-based platform for detection of microRNAs.RNA (New York, N.Y.) · 2011Article
- Road blocks on paleogenomes--polymerase extension profiling reveals the frequency of blocking lesions in ancient DNA.Nucleic acids research · 2010Article
- Functional viral metagenomics and the next generation of molecular tools.Trends in microbiology · 2010Review
- Classification of Myoviridae bacteriophages using protein sequence similarity.BMC microbiology · 2009Article
- Identification of a tail assembly gene cluster from deep-sea thermophilic bacteriophage GVE2.Virus genes · 2009Article
- Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA.Nucleic acids research · 2008Article
- Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4.The Biochemical journal · 2006Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of genes with potential use in biotechnology. We have also demonstrated the existence of thermostable counterparts of previously known bacteriophage enzymes. Here we describe a thermostable RNA ligase 1 from the thermophilic bacteriophage RM378 that infects the thermophilic eubacterium Rhodothermus marinus. The RM378 RNA ligase 1 has a temperature optimum of 60-64 degrees C and it ligates both RNA and single-stranded DNA. Its thermostability and ability to work under conditions of high temperature where nucleic acid secondary structures are removed makes it an ideal enzyme for RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE), and other RNA and DNA ligation applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.