ArticleNucleic acids research2003
Molecular genetic analysis of chloroplast gene promoters dependent on SIG2, a nucleus-encoded sigma factor for the plastid-encoded RNA polymerase, in Arabidopsis thaliana.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 104 citations in OpenAlex.
- Rubisco functional integrity requires a dedicated three-step maturation pathway.Science advances · 2026Article
- Regreening mechanisms in cucumber: insights from a CsSIG2 mutation affecting chloroplast development.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- An interplay between bZIP16, bZIP68, and GBF1 regulates nuclear photosynthetic genes during photomorphogenesis in Arabidopsis.The New phytologist · 2023Article
- Complete chloroplast genome molecular structure, comparative and phylogenetic analyses of Sphaeropteris lepifera of Cyatheaceae family: a tree fern from China.Scientific reports · 2023Article
- High-resolution map of plastid-encoded RNA polymerase binding patterns demonstrates a major role of transcription in chloroplast gene expression.The Plant journal : for cell and molecular biology · 2022Article
- An Argon-Ion-Induced Pale Green Mutant ofPlants (Basel, Switzerland) · 2021Article
- A pentatricopeptide repeat protein DUA1 interacts with sigma factor 1 to regulate chloroplast gene expression in Rice.Photosynthesis research · 2021Article
- Plastid Gene Transcription: An Update on Promoters and RNA Polymerases.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Functions of Chloroplast Glutamyl-tRNA in Translation and Tetrapyrrole Biosynthesis.Plant physiology · 2020Article
- Sigma factor 1 in chloroplast gene transcription and photosynthetic light acclimation.Journal of experimental botany · 2020Article
- Systematic sequencing of chloroplast transcript termini from Arabidopsis thaliana reveals >200 transcription initiation sites and the extensive imprints of RNA-binding proteins and secondary structures.Nucleic acids research · 2019Article
- The caseinolytic protease complex component CLPC1 in Arabidopsis maintains proteome and RNA homeostasis in chloroplasts.BMC plant biology · 2018Article
- LLM-Domain B-GATA Transcription Factors Play Multifaceted Roles in Controlling Greening in Arabidopsis.The Plant cell · 2018Article
- Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana.Nature communications · 2018Article
- SKL1 Is Essential for Chloroplast Development in Arabidopsis.Frontiers in plant science · 2018Article
- A quantitative model of the phytochrome-PIF light signalling initiating chloroplast development.Scientific reports · 2017Article
- Article
- Lethal albinic seedling, encoding a threonyl-tRNA synthetase, is involved in development of plastid protein synthesis system in rice.Plant cell reports · 2017Article
- Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor.The New phytologist · 2017Article
- Leaf Variegation of Thylakoid Formation1 Is Suppressed by Mutations of Specific σ-Factors in Arabidopsis.Plant physiology · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most photosynthesis-related genes in mature chloroplasts are transcribed by a eubacterial-type RNA polymerase (PEP) whose core subunits are encoded by the plastid genome. It has been shown previously that six putative nuclear genes (SIG1 to SIG6) encode promoter-specificity factors for PEP in Arabidopsis thaliana, and we isolated a T-DNA insertion line of SIG2 (sig2-1 mutant) that manifests aberrant chloroplast development. With the use of S1 nuclease protection and primer extension analyses, we have now characterized the SIG2-dependent chloroplast promoters in A.thaliana. The amounts of transcripts derived from one of the multiple psbD promoters (psbD -256) and from the promoters of two tRNA genes (trnE-UUC and trnV-UAC) were markedly and specifically decreased in the sig2-1 mutant. The abundance of these transcripts was restored to wild-type levels by introduction into the mutant of a SIG2 transgene. The recombinant SIG2 protein mixed with Escherichia coli core RNA polymerase could bind to a DNA fragment that contains the SIG2-dependent psbD -256, trnE-UUC or trnV-UAC promoter. Sequences similar to those of the -35 and -10 promoter elements of E.coli were identified in the regions of the SIG2-dependent chloroplast genes upstream of the transcription initiation sites.
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