ArticleProceedings of the National Academy of Sciences of the United States of America1992
Ovariectomy selectively reduces the concentration of transforming growth factor beta in rat bone: implications for estrogen deficiency-associated bone loss.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 2 syntheses or guidelines pooled it, 128 citations in OpenAlex.
- Clinical Data for Parametrization ofFrontiers in bioengineering and biotechnology · 2022Pooled it
- Pooled it
- Changing landscape of hematopoietic and mesenchymal cells and their interactions during aging and in age-related skeletal pathologies.Mechanisms of ageing and development · 2025Review
- Two murine models of sepsis: immunopathological differences between the sexes-possible role of TGFβ1 in female resistance to endotoxemia.Biological research · 2023Article
- Effects of a Whole Plant Extract ofPlants (Basel, Switzerland) · 2022Article
- Distinct pathological changes of osteochondral units in early OVX-OA involving TGF-β signaling.Frontiers in endocrinology · 2022Article
- Agonists and knockdown of estrogen receptor β differentially affect invasion of triple-negative breast cancer cells in vitro.BMC cancer · 2016Article
- Ovariectomy differential influence on some hemostatic markers of mice and rats.Experimental animals · 2015Article
- Article
- Poor histological healing of a femoral fracture following 12 months of oestrogen deficiency in rats.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2013Article
- Osteoimmunology and the influence of pro-inflammatory cytokines on osteoclasts.Biochemia medica · 2013Review
- Perspective on post-menopausal osteoporosis: establishing an interdisciplinary understanding of the sequence of events from the molecular level to whole bone fractures.Journal of the Royal Society, Interface · 2010Review
- Estrogen-TGFbeta cross-talk in bone and other cell types: role of TIEG, Runx2, and other transcription factors.Journal of cellular biochemistry · 2008Review
- The effect of orchidectomy on thyroid C cells and bone histomorphometry in middle-aged rats.Histochemistry and cell biology · 2007Article
- An IL-7-dependent rebound in thymic T cell output contributes to the bone loss induced by estrogen deficiency.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Estrogen prevents bone loss through transforming growth factor beta signaling in T cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- RANK ligand and the regulation of skeletal remodeling.The Journal of clinical investigation · 2003Article
- Hormonal regulation of physiological cell turnover and apoptosis.Cell and tissue research · 2000Review
- Transforming growth factor-beta1 modulates chondrocyte responsiveness to 17beta-estradiol.Endocrine · 1999Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Previous work showed that production of transforming growth factor beta (TGF-beta) by osteoblast-like rat UMR 106 cells was increased by 17 beta-estradiol at physiological concentrations. To determine whether ovariectomy alters the concentration of TGF-beta in rat long bones, female Sprague-Dawley rats were either sham-operated (n = 19) or ovariectomized (n = 19), pair-fed a semisynthetic diet for 6 weeks, and sacrificed. Tibial and femoral diaphyses were removed and extracted by demineralization. Ovariectomy lowered serum estrogen; did not alter body weight, serum magnesium, or serum 1,25-dihydroxyvitamin D; and produced only modest differences in serum calcium and phosphate concentrations. Hydroxyproline was higher and extractable protein was lower in bones from ovariectomized rats than in bones from sham-operated rats; calcium content did not differ between the two groups of animals. Ovariectomy lowered the concentration of TGF-beta in bone but did not change the concentration of insulin-like growth factors I or II compared with values in bone from control animals. The reduction of bone TGF-beta was evident 6 weeks after surgery but not at 3 weeks. Treatment of ovariectomized rats with estrogen eliminated the TGF-beta deficit. To determine whether 17 beta-estradiol increased TGF-beta production by normal bone cells, mouse osteoblasts were treated for 2 days with 17 beta-estradiol. The production of TGF-beta was increased almost 2-fold by 1 nM 17 beta-estradiol, and short-term treatment stimulated the intracellular accumulation of TGF-beta 1 mRNA. We conclude that ovariectomy reduces deposition of TGF-beta in rat bone and that diminished skeletal TGF-beta could play a role in the pathogenesis of bone loss, fractures, and microfractures that occur in estrogen-deficient states. Our results support the possibility that estrogen and bone TGF-beta may be necessary for normal maintenance of the skeleton in female rats.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.