ArticleProceedings of the National Academy of Sciences of the United States of America1992
Metabolic engineering of medicinal plants: transgenic Atropa belladonna with an improved alkaloid composition.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 310 citations in OpenAlex.
- A Comprehensive Review on the Biosynthesis of Tropane Alkaloids.Molecules (Basel, Switzerland) · 2026Review
- Enhancement of specialized metabolites using CRISPR/Cas gene editing technology in medicinal plants.Frontiers in plant science · 2024Review
- Biotechnological Approaches on Engineering Medicinal Tropane Alkaloid Production in Plants.Frontiers in plant science · 2022Review
- Production of bioactive plant secondary metabolites through in vitro technologies-status and outlook.Applied microbiology and biotechnology · 2021Review
- Effect of Polyploidy Induction on Natural Metabolite Production in Medicinal Plants.Biomolecules · 2021Review
- Development ofInternational journal of molecular sciences · 2021Article
- Improvement of phytochemical production by plant cells and organ culture and by genetic engineering.Plant cell reports · 2019Review
- Influence of nano-zinc oxide on tropane alkaloid production,Engineering in life sciences · 2019Article
- Enhancing Tropane Alkaloid Production Based on the Functional Identification of Tropine-Forming Reductase inFrontiers in plant science · 2017Article
- Tropane and Granatane Alkaloid Biosynthesis: A Systematic Analysis.Molecules (Basel, Switzerland) · 2016Review
- Exploring the Metabolic Stability of Engineered Hairy Roots after 16 Years Maintenance.Frontiers in plant science · 2016Article
- Transcriptome exploration for further understanding of the tropane alkaloids biosynthesis in Anisodus acutangulus.Molecular genetics and genomics : MGG · 2015Article
- Tilting Plant Metabolism for Improved Metabolite Biosynthesis and Enhanced Human Benefit.Molecules (Basel, Switzerland) · 2015Review
- Methyl jasmonate-elicited transcriptional responses and pentacyclic triterpene biosynthesis in sweet basil.Plant physiology · 2014Article
- Article
- Microbial synthetic biology for human therapeutics.Systems and synthetic biology · 2012Article
- Co-expression of AaPMT and AaTRI effectively enhances the yields of tropane alkaloids in Anisodus acutangulus hairy roots.BMC biotechnology · 2011Article
- Opportunities in metabolic engineering to facilitate scalable alkaloid production.Nature chemical biology · 2009Article
- Metabolic reprogramming of periwinkle plant culture.Nature chemical biology · 2009Article
- Application of metabolic engineering to the production of scopolamine.Molecules (Basel, Switzerland) · 2008Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tropane alkaloid scopolamine is a medicinally important anticholinergic drug present in several solanaceous plants. Hyoscyamine 6 beta-hydroxylase (EC 1.14.11.11) catalyzes the oxidative reactions in the biosynthetic pathway leading from hyoscyamine to scopolamine. We introduced the hydroxylase gene from Hyoscyamus niger under the control of the cauliflower mosaic virus 35S promoter into hyoscyamine-rich Atropa belladonna by the use of an Agrobacterium-mediated transformation system. A transgenic plant that constitutively and strongly expressed the transgene was selected, first by screening for kanamycin resistance and then by immunoscreening leaf samples with an antibody specific for the hydroxylase. In the primary transformant and its selfed progeny that inherited the transgene, the alkaloid contents of the leaf and stem were almost exclusively scopolamine. Such metabolically engineered plants should prove useful as breeding materials for obtaining improved medicinal components.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.