ArticleJournal of the American Society for Mass Spectrometry2003
The role of acidic residues and of sodium ion adduction on the gas-phase H/D exchange of peptides and peptide dimers.
Article in Journal of the American Society for Mass Spectrometry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Structural Analysis of the Effect of a Dual-FLAG Tag on Transthyretin.Biochemistry · 2020Article
- Mass Spectral Detection of Forward- and Reverse-Hydrogen/Deuterium Exchange Resulting from Residual Solvent Vapors in Electrospray Sources.Journal of the American Society for Mass Spectrometry · 2018Article
- Gas-phase acidities of cysteine-polyglycine peptides: the effect of the cysteine position.Journal of the American Society for Mass Spectrometry · 2010Article
- Gas-phase H/D exchange and collision cross sections of hemoglobin monomers, dimers, and tetramers.Journal of the American Society for Mass Spectrometry · 2009Article
- Isomeric differentiation of green tea catechins using gas-phase hydrogen/deuterium exchange reactions.Journal of the American Society for Mass Spectrometry · 2007Article
- Gas-phase hydrogen/deuterium exchange of 5'- and 3'-mononucleotides in a quadrupole ion trap: exploring the role of conformation and system energy.Journal of the American Society for Mass Spectrometry · 2007Article
- Observation of zwitterion formation in the gas-phase H/D-exchange with CH(3)OD: solution-phase structures in the gas phase.Journal of the American Society for Mass Spectrometry · 2007Article
- Enhanced mixture analysis of poly(ethylene glycol) using high-field asymmetric waveform ion mobility spectrometry combined with fourier transform ion cyclotron resonance mass spectrometry.Analytical chemistry · 2006Article
- Multidimensional separations of ubiquitin conformers in the gas phase: relating ion cross sections to H/D exchange measurements.Journal of the American Society for Mass Spectrometry · 2005Article
- Direct correlation of the crystal structure of proteins with the maximum positive and negative charge states of gaseous protein ions produced by electrospray ionization.Journal of the American Society for Mass Spectrometry · 2005Article
- Evaluation of ion mobility spectroscopy for determining charge-solvated versus salt-bridge structures of protonated trimers.Journal of the American Society for Mass Spectrometry · 2005Article
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Authors and funding
3 authors.
Funding
Abstract
Gas-phase H/D exchange is widely used for characterizing the structure of ions. However, many structural parameters that affect the rate of H/D exchange are poorly understood, which complicates the interpretation of experimental data. Here, the effects of sodium ion adduction on the rate of H/D exchange with D2O for a series of peptides and peptide dimers with varying numbers of acidic residues are described. The maximum number of sodium ion adducts that can be accommodated by the peptides and peptide dimers in this study is N + 1, where N is the number of free carboxylic acid groups. The formation of methyl-esters at all carboxylic acid groups, or the replacement of all the acidic hydrogens with sodium ions, effectively shuts down H/D exchange with D2O. In contrast, both the rate and the extent of H/D exchange with D2O are increased for most of the peptides and peptide dimers by the adduction of an intermediate number of sodium ions. These results are consistent with the H/D exchange occurring via a salt-bridge mechanism and show that the presence of two carboxylic acid groups is much better than one. The results with peptide dimers also indicate that surface accessibility may not be a dominant factor in the extent of H/D exchange for these ions.
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