ArticleMolecular microbiology2003
Distribution of split DnaE inteins in cyanobacteria.
Article in Molecular microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 70 citations in OpenAlex.
- Regulation of N-terminal cleavage and splicing in mini-Ter DnaE-3 intein by E23G and R50Q mutations.Biotechnology letters · 2026Article
- Pre-mRNA splicing and its regulation in microalgae and cyanobacteria.Advanced biotechnology · 2025Review
- Characterization of NiCas12b for In Vivo Genome Editing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Article
- Neighboring inteins interfere with one another's homing capacity.PNAS nexus · 2023Article
- A Convenient Self-Removing Affinity Tag Method for the Simple Purification of Tagless Recombinant Proteins.Current protocols · 2023Article
- Extending AAV Packaging Cargo through Dual Co-Transduction: Efficient Protein Trans-Splicing at Low Vector Doses.International journal of molecular sciences · 2023Article
- Inteins as Drug Targets and Therapeutic Tools.Frontiers in molecular biosciences · 2022Review
- Comparative analysis reveals distinctive genomic features of Taiwan hot-spring cyanobacteriumFrontiers in microbiology · 2022Article
- A designed fusion tag for soluble expression and selective separation of extracellular domains of fibroblast growth factor receptors.Scientific reports · 2021Article
- A mesophilic cysteine-less split intein for proteinProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology.Microorganisms · 2019Review
- Biotechnological Applications of Protein Splicing.Current protein & peptide science · 2019Review
- Genomic Characteristics ofFrontiers in microbiology · 2019Article
- Inteins: Localized Distribution, Gene Regulation, and Protein Engineering for Biological Applications.Microorganisms · 2018Review
- A promiscuous split intein with expanded protein engineering applications.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A molecular engineering toolbox for the structural biologist.Quarterly reviews of biophysics · 2017Review
- Nature's recipe for splitting inteins.Protein engineering, design & selection : PEDS · 2014Review
- Protein splicing: how inteins escape from precursor proteins.The Journal of biological chemistry · 2014Review
- Structural and dynamical features of inteins and implications on protein splicing.The Journal of biological chemistry · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inteins are genetic elements found inside the coding regions of different host proteins and are translated in frame with them. The intein-encoded protein region is removed by an autocatalytic protein-splicing reaction that ligates the host protein flanks with a peptide bond. This reaction can also occur in trans with the intein and host protein split in two. After translation of the two genes, the two intein parts ligate their flanking protein parts to each other, producing the mature protein. Naturally split inteins are only known in the DNA polymerase III alpha subunit (polC or dnaE gene) of a few cyanobacteria. Analysing the phylogenetic distribution and probable genetic propagation mode of these split inteins, we conclude that they are genetically fixed in several large cyanobacterial lineages. To test our hypothesis, we sequenced parts of the dnaE genes from five diverse cyanobacteria and found all species to have the same type of split intein. Our results suggest the occurrence of a genetic rearrangement in the ancestor of a large division of cyanobacteria. This event fixed the dnaE gene in a unique two-genes one-protein configuration in the progenitor of many cyanobacteria. Our hypothesis, findings and the cloning procedure that we established allow the identification and acquisition of many naturally split inteins. Having a large and diverse repertoire of these unique inteins will enable studies of their distinct activity and enhance their use in biotechnology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.