ArticleBiopolymers2003
Effects of metallic silver island films on resonance energy transfer between N,N'-(dipropyl)-tetramethyl- indocarbocyanine (Cy3)- and N,N'-(dipropyl)-tetramethyl- indodicarbocyanine (Cy5)-labeled DNA.
Article in Biopolymers, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Fluorescence Resonance Energy Transfer Characterization of DNA Wrapping in Closed and Open Escherichia coli RNA Polymerase-λP(R) Promoter Complexes.Biochemistry · 2016Article
- Extending the range of FRET--the Monte Carlo study of the antenna effect.Journal of molecular modeling · 2013Article
- Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores: enhanced transfer in the presence of multiple acceptors.Journal of biomedical optics · 2012Article
- Effect of plasmonic nanostructures and nanofilms on fluorescence resonance energy transfer.Journal of biophotonics · 2009Article
- Plasmon-controlled fluorescence towards high-sensitivity optical sensing.Advances in biochemical engineering/biotechnology · 2009Review
- Review
- Decreasing photobleaching by silver island films: application to muscle.Analytical biochemistry · 2007Article
- Metal enhanced fluorescence solution-based sensing platform 2: fluorescent core-shell Ag@SiO2 nanoballs.Journal of fluorescence · 2007Article
- Fluorescent core-shell Ag@SiO2 nanocomposites for metal-enhanced fluorescence and single nanoparticle sensing platforms.Journal of the American Chemical Society · 2007Article
- Metal-enhanced fluorescence from CdTe nanocrystals: a single-molecule fluorescence study.Journal of the American Chemical Society · 2006Article
- Langmuir-Blodgett monolayers of long-chain NBD derivatives on silver island films: Well-organized probe layer for the metal-enhanced fluorescence studies.The journal of physical chemistry. B · 2006Article
- Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films.Biochemical and biophysical research communications · 2005Article
- Metal-enhanced fluorescence immunoassays using total internal reflection and silver island-coated surfaces.Analytical biochemistry · 2004Article
- First observation of surface plasmon-coupled electrochemiluminescence.Chemical physics letters · 2004Article
- Surface Plasmon-Coupled Emission with Gold Films.The journal of physical chemistry. B · 2004Article
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Authors and funding
4 authors.
Funding
Abstract
Resonance energy transfer (RET) is typically limited to distances below 60 A, which can be too short for some biomedical assays. We examined a new method for increasing the RET distances by placing donor- and acceptor-labeled DNA oligomers between two slides coated with metallic silver particles. A N,N'-(dipropyl)-tetramethylindocarbocyanine donor and a N,N'-(dipropyl)-tetramethylindodicarbocyanine acceptor were covalently bound to opposite 5' ends of complementary 23 base pair DNA oligomers. The transfer efficiency was 25% in the absence of silver particles or if only one slide was silvered, and it increased to an average value near 64% between two silvered slides. The average value of the Forster distance increased from 58 to 77 A. The energy transfer data were analyzed with a model assuming two populations of donor-acceptor pairs: unaffected and affected by silver island films. In an affected fraction of about 28%, the apparent energy transfer efficiency is near 87% and the Forster distance increases to 119 A. These results suggest the use of metallic silver particles to increase the distances over which RET occurs in biomedical and biotechnology assays.
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