ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2004
Characterization and molecular genetic mapping of microsatellite loci in pepper.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 201 citations in OpenAlex.
- Identification and characterization of Capsicum mutants using, biochemical, physiological, and single sequence repeat (SSR) markers.Journal, genetic engineering & biotechnology · 2024Article
- Genetic Variation Study of Several Romanian Pepper (International journal of molecular sciences · 2024Article
- Ecology, genetic diversity, and population structure among commercial varieties and local landraces ofFrontiers in plant science · 2024Article
- The bs5 allele of the susceptibility gene Bs5 of pepper (Capsicum annuum L.) encoding a natural deletion variant of a CYSTM protein conditions resistance to bacterial spot disease caused by Xanthomonas species.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
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- Chile Pepper (Frontiers in plant science · 2022Review
- Molecular mapping of dominant gene responsible for leaf curl virus resistance in chilli pepper (3 Biotech · 2020Article
- Target sequencing reveals genetic diversity, population structure, core-SNP markers, and fruit shape-associated loci in pepper varieties.BMC plant biology · 2019Article
- Development and Application of InDel Markers for Capsicum spp. Based on Whole-Genome Re-Sequencing.Scientific reports · 2019Article
- Detection of quantitative trait loci for capsanthin content in pepper (Breeding science · 2019Article
- Whole Genome Resequencing of Capsicum baccatum and Capsicum annuum to Discover Single Nucleotide Polymorphism Related to Powdery Mildew Resistance.Scientific reports · 2018Article
- Mapping of fruit length related QTLs in interspecific cross (Breeding science · 2018Article
- Conservation and Dispersion of Genes Conferring Resistance to Tomato Begomoviruses between Tomato and Pepper Genomes.Frontiers in plant science · 2017Article
- A comprehensive characterization of simple sequence repeats in pepper genomes provides valuable resources for marker development in Capsicum.Scientific reports · 2016Article
- An InDel-based linkage map of hot pepper (Molecular breeding : new strategies in plant improvement · 2015Article
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- Article
- Multiple lines of evidence for the origin of domesticated chili pepper, Capsicum annuum, in Mexico.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- New insights into Capsicum spp relatedness and the diversification process of Capsicum annuum in Spain.PloS one · 2014Article
- De novo transcriptome assembly and novel microsatellite marker information in Capsicum annuum varieties Saengryeg 211 and Saengryeg 213.Botanical studies · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microsatellites or simple sequence repeats are highly variable DNA sequences that can be used as informative markers for the genetic analysis of plants and animals. For the development of microsatellite markers in Capsicum, microsatellites were isolated from two small-insert genomic libraries and the GenBank database. Using five types of oligonucleotides, (AT)(15), (GA)(15), (GT)(15), (ATT)(10) and (TTG)(10), as probes, positive clones were isolated from the genomic libraries, and sequenced. Out of 130 positive clones, 77 clones showed microsatellite motifs, out of which 40 reliable microsatellite markers were developed. (GA)(n) and (GT)(n) sequences were found to occur most frequently in the pepper genome, followed by (TTG)(n) and (AT)(n). Additional 36 microsatellite primers were also developed from GenBank and other published data. To measure the information content of these markers, the polymorphism information contents (PICs) were calculated. Capsicum microsatellite markers from the genomic libraries have shown a high level of PIC value, 0.76, twice the value for markers from GenBank data. Forty six microsatellite loci were placed on the SNU-RFLP linkage map, which had been derived from the interspecific cross between Capsicum annuum "TF68" and Capsicum chinense "Habanero". The current "SNU2" pepper map with 333 markers in 15 linkage groups contains 46 SSR and 287 RFLP markers covering 1,761.5 cM with an average distance of 5.3 cM between markers.
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