ArticleMolecular and cellular biology2003
Affinity purification of specific chromatin segments from chromosomal loci in yeast.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Nucleosomes and IDRs suppress promiscuous GCN4 binding on minichromosomes.Nature structural & molecular biology · 2026Article
- Single molecule MATAC-seq reveals key determinants of DNA replication origin efficiency.Nucleic acids research · 2023Article
- Review
- Dissecting Locus-Specific Chromatin Interactions by CRISPR CAPTURE.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Analysis of Yeast RNAP I Transcription of Nucleosomal Templates In Vitro.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Purification and enrichment of specific chromatin loci.Nature methods · 2020Review
- Nucleosomal proofreading of activator-promoter interactions.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Proximity Labeling Techniques to Study Chromatin.Frontiers in genetics · 2020Review
- Genome-wide reconstitution of chromatin transactions reveals that RSC preferentially disrupts H2AZ-containing nucleosomes.Genome research · 2019Article
- CAPTURE: In Situ Analysis of Chromatin Composition of Endogenous Genomic Loci by Biotinylated dCas9.Current protocols in molecular biology · 2018Article
- Design principles for nuclease-deficient CRISPR-based transcriptional regulators.FEMS yeast research · 2018Review
- dCas9-targeted locus-specific protein isolation method identifies histone gene regulators.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Toehold-enhanced LNA probes for selective pull down and single-molecule analysis of native chromatin.Scientific reports · 2017Article
- Chromatin potentiates transcription.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP.Journal of visualized experiments : JoVE · 2016Article
- Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies.Gene regulation and systems biology · 2016Review
- End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter.Nature communications · 2015Article
- Article
- Nucleosomal promoter variation generates gene expression noise.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
Single-copy gene and promoter regions have been excised from yeast chromosomes and have been purified as chromatin by conventional and affinity methods. Promoter regions isolated in transcriptionally repressed and activated states maintain their characteristic chromatin structures. Gel filtration analysis establishes the uniformity of the transcriptionally activated state. Activator proteins interact in the manner anticipated from previous studies in vivo. This work opens the way to the direct study of specific gene regions of eukaryotic chromosomes in diverse functional and structural states.
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