ArticleMolecular and cellular biology2003
Myocardin expression is regulated by Nkx2.5, and its function is required for cardiomyogenesis.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 111 citations in OpenAlex.
- Myeloid Ecotropic Viral Integration Site 1 Promotes Vascular Smooth Muscle Differentiation through Activation of Myocardin.bioRxiv : the preprint server for biology · 2026Article
- Spatial expression of myocardin protein in normal and disease states.American journal of physiology. Cell physiology · 2026Article
- Posttranscriptional Regulation by Proteins and Noncoding RNAs.Advances in experimental medicine and biology · 2024Article
- SMYD2 regulates vascular smooth muscle cell phenotypic switching and intimal hyperplasia via interaction with myocardin.Cellular and molecular life sciences : CMLS · 2023Article
- SMYD2 Regulates Vascular Smooth Muscle Cell Phenotypic Switching and Intimal Hyperplasia via Interaction with Myocardin.Research square · 2023Article
- QKI is a critical pre-mRNA alternative splicing regulator of cardiac myofibrillogenesis and contractile function.Nature communications · 2021Article
- Crystal Structures of Ternary Complexes of MEF2 and NKX2-5 Bound to DNA Reveal a Disease Related Protein-Protein Interaction Interface.Journal of molecular biology · 2020Article
- Lineage-specific reorganization of nuclear peripheral heterochromatin and H3K9me2 domains.Development (Cambridge, England) · 2019Article
- Curcumin enhances vascular contractility via induction of myocardin in mouse smooth muscle cells.Acta pharmacologica Sinica · 2017Article
- Circular RNA ciRS-7-A Promising Prognostic Biomarker and a Potential Therapeutic Target in Colorectal Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2017Article
- Yes-Associated Protein Inhibits Transcription of Myocardin and Attenuates Differentiation of Vascular Smooth Muscle Cell from Cardiovascular Progenitor Cell Lineage.Stem cells (Dayton, Ohio) · 2017Article
- Ezh2 restricts the smooth muscle lineage during mouse lung mesothelial development.Development (Cambridge, England) · 2016Article
- Cadherin-11 is a novel regulator of extracellular matrix synthesis and tissue mechanics.Journal of cell science · 2016Article
- Article
- Investigation of Somatic NKX2-5 Mutations in Chinese Children with Congenital Heart Disease.International journal of medical sciences · 2015Article
- Myocardin in biology and disease.Journal of biomedical research · 2015Review
- Generation of a Cre knock-in into the Myocardin locus to mark early cardiac and smooth muscle cell lineages.Genesis (New York, N.Y. : 2000) · 2014Article
- The functional diversity of essential genes required for mammalian cardiac development.Genesis (New York, N.Y. : 2000) · 2014Review
- Cell division cycle 7 mediates transforming growth factor-β-induced smooth muscle maturation through activation of myocardin gene transcription.The Journal of biological chemistry · 2013Article
- Evolutionary conservation of Nkx2.5 autoregulation in the second heart field.Developmental biology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Nkx2.5 (also known as Csx) is an evolutionarily conserved cardiac transcription factor of the homeobox gene family. Nkx2.5 is required for early heart development, since Nkx2.5-null mice die before completion of cardiac looping. To identify genes regulated by Nkx2.5 in the developing heart, we performed subtractive hybridization by using RNA isolated from wild-type and Nkx2.5-null hearts at embryonic day 8.5. We isolated a mouse cDNA encoding myocardin A, which is an alternative spliced isoform of myocardin and the most abundant isoform in the heart from embryo to adult. The expression of myocardin A and myocardin was markedly downregulated in Nkx2.5-null mouse hearts. Transient-cotransfection analysis showed that Nkx2.5 transactivates the myocardin promoter. Inhibition of myocardin function in the teratocarcinoma cell line P19CL6 prevented differentiation into cardiac myocytes after dimethyl sulfoxide treatment. Myocardin A transactivated the promoter of the atrial natriuretic factor gene through the serum response element, which was augmented by bone morphogenetic protein 2 and transforming growth factor beta-activated kinase 1. These results suggest that myocardin expression is regulated by Nkx2.5 and that its function is required for cardiomyogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.