ArticleThe Journal of physiology2004
Sensitivity limits for voltage control of P2Y receptor-evoked Ca2+ mobilization in the rat megakaryocyte.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Charge Movements and Conformational Changes: Biophysical Properties and Physiology of Voltage-Dependent GPCRs.Biomolecules · 2024Review
- Functional consequences of a rare human serotonergic 5-HTFrontiers in pharmacology · 2023Article
- G Protein-Coupled Receptors Regulated by Membrane Potential.International journal of molecular sciences · 2022Review
- The activity of the serotonergic 5-HTThe Journal of biological chemistry · 2022Article
- Voltage dependence of the cannabinoid CB1 receptor.Frontiers in pharmacology · 2022Article
- Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches.Molecules (Basel, Switzerland) · 2020Review
- Intracellular Transfer of NaStructure (London, England : 1993) · 2018Article
- Membrane potentials regulating GPCRs: insights from experiments and molecular dynamics simulations.Current opinion in pharmacology · 2016Review
- Structural Mechanisms of Voltage Sensing in G Protein-Coupled Receptors.Structure (London, England : 1993) · 2016Article
- Blood cells: an historical account of the roles of purinergic signalling.Purinergic signalling · 2015Review
- ATP inhibits Ins(1,4,5)P3-evoked Ca2+ release in smooth muscle via P2Y1 receptors.Journal of cell science · 2012Article
- Depolarization induces a conformational change in the binding site region of the M2 muscarinic receptor.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Direct voltage control of endogenous lysophosphatidic acid G-protein-coupled receptors in Xenopus oocytes.The Journal of physiology · 2010Article
- ATP released by electrical stimuli elicits calcium transients and gene expression in skeletal muscle.The Journal of biological chemistry · 2009Article
- Novel consequences of voltage-dependence to G-protein-coupled P2Y1 receptors.British journal of pharmacology · 2008Article
- Glutamate mediates platelet activation through the AMPA receptor.The Journal of experimental medicine · 2008Article
- Modulation of Gq-protein-coupled inositol trisphosphate and Ca2+ signaling by the membrane potential.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
G-protein-coupled receptor signalling has been suggested to be voltage dependent in a number of cell types; however, the limits of sensitivity of this potentially important phenomenon are unknown. Using the non-excitable rat megakaryocyte as a model system, we now show that P2Y receptor-evoked Ca2+ mobilization is controlled by membrane voltage in a graded and bipolar manner without evidence for a discrete threshold potential. Throughout the range of potentials studied, the peak increase in intracellular Ca2+ concentration ([Ca2+]i) in response to depolarization was always larger than the maximal reduction in [Ca2+]i following an equivalent amplitude hyperpolarization. Significant [Ca2+]i increases were observed in response to small amplitude (< 5 mV, 5 s duration) or short duration (25 ms, 135 mV) depolarizations. Individual cardiac action potential waveforms were also able to repeatedly potentiate P2Y receptor-evoked Ca2+ release and the response to trains of normally paced stimuli fused to generate prolonged [Ca2+]i increases. Furthermore, elevation of the temperature to physiological levels (36 degrees C) resulted in a more sustained depolarization-evoked Ca2+ increase compared with more transient or oscillatory responses at 20-24 degrees C. The ability of signalling via a G-protein-coupled receptor to be potentiated by action potential waveforms and small amplitude depolarizations has broad implications in excitable and non-excitable tissues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.