ArticleJournal of bacteriology2003
Pathways leading from BarA/SirA to motility and virulence gene expression in Salmonella.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed, 166 citations in OpenAlex.
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- Making a Pathogen? Evaluating the Impact of Protist Predation on the Evolution of Virulence in Serratia marcescens.Genome biology and evolution · 2024Article
- Persistent Salmonella infections in humans are associated with mutations in the BarA/SirA regulatory pathway.Cell host & microbe · 2024Article
- From intestine to beyond:Open biology · 2024Article
- A Novel Role of the Two-Component System Response Regulator UvrY in EnterohemorrhagicInternational journal of molecular sciences · 2023Article
- Mitigating the attachment of Salmonella Infantis on isolated poultry skin with cetylpyridinium chloride.PloS one · 2023Article
- Envelope Stress Activates Expression of the Twin Arginine Translocation (Tat) System in Salmonella.Microbiology spectrum · 2022Article
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- An incoherent feedforward loop formed by SirA/BarA, HilE and HilD is involved in controlling the growth cost of virulence factor expression by Salmonella Typhimurium.PLoS pathogens · 2021Article
- HilE is required for synergistic activation of SPI-1 gene expression in Salmonella enterica serovar Typhimurium.BMC microbiology · 2021Article
- UvrY is required for the full virulence ofVirulence · 2020Article
- New Roles for Two-Component System Response Regulators ofMicroorganisms · 2020Article
- The phosphorelay BarA/SirA activates the non-cognate regulator RcsB in Salmonella enterica.PLoS genetics · 2020Article
- Differential Regulation of CsrC and CsrB by CRP-cAMP inFrontiers in microbiology · 2020Article
- Synergistic action of SPI-1 gene expression in Salmonella enterica serovar typhimurium through transcriptional crosstalk with the flagellar system.BMC microbiology · 2019Article
- Salmonella invasion is controlled through the secondary structure of the hilD transcript.PLoS pathogens · 2019Article
32 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The barA and sirA genes of Salmonella enterica serovar Typhimurium encode a two-component sensor kinase and a response regulator, respectively. This system increases the expression of virulence genes and decreases the expression of motility genes. In this study, we examined the pathways by which SirA affects these genes. We found that the master regulator of flagellar genes, flhDC, had a positive regulatory effect on the primary regulator of intestinal virulence determinants, hilA, but that hilA had no effect on flhDC. SirA was able to repress flhDC in a hilA mutant and activate hilA in an flhDC mutant. Therefore, although the flhDC and hilA regulatory cascades interact, sirA affects each of them independently. A form of BarA lacking the two N-terminal membrane-spanning domains, BarA198, autophosphorylates in the presence of ATP and transfers the phosphate to purified SirA. Phosphorylated SirA was found to directly bind the hilA and hilC promoters in gel mobility shift assays but not the flhD, fliA, hilD, and invF promoters. Given that the CsrA/csrB system is known to directly affect flagellar gene expression, we tested the hypothesis that SirA affects flagellar gene expression indirectly by regulating csrA or csrB. The sirA gene did not regulate csrA but did activate csrB expression. Consistent with these results, phosphorylated SirA was found to directly bind the csrB promoter but not the csrA promoter. We propose a model in which SirA directly activates virulence expression via hilA and hilC while repressing the flagellar regulon indirectly via csrB.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.