ArticleBiophysical journal2003
Electrophysiological analysis of the yeast V-type proton pump: variable coupling ratio and proton shunt.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 62 citations in OpenAlex.
- Multiscale kinetic analysis of proteins.Current opinion in structural biology · 2022Review
- Modulation of the HFrontiers in molecular biosciences · 2022Review
- The Plant V-ATPase.Frontiers in plant science · 2022Review
- Inside the Insulin Secretory Granule.Metabolites · 2021Review
- CLIC2α Chloride Channel Orchestrates Immunomodulation of Hemocyte Phagocytosis and Bactericidal Activity in Crassostrea gigas.iScience · 2020Article
- A systems-biology approach to molecular machines: Exploration of alternative transporter mechanisms.PLoS computational biology · 2020Article
- Exploring the Therapeutic Potential of Membrane Transport Proteins: Focus on Cancer and Chemoresistance.Cancers · 2020Review
- EmrE reminds us to expect the unexpected in membrane transport.The Journal of general physiology · 2020Article
- Regulation of Amino Acid Transport in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2019Review
- Article
- Disorders of lysosomal acidification-The emerging role of v-ATPase in aging and neurodegenerative disease.Ageing research reviews · 2016Review
- Acid-inducible proton influx currents in the plasma membrane of murine osteoclast-like cells.Pflugers Archiv : European journal of physiology · 2016Article
- Yeast V-ATPase Proteolipid Ring Acts as a Large-conductance Transmembrane Protein Pore.Scientific reports · 2016Article
- Modeling the vacuolar storage of malate shed lights on pre- and post-harvest fruit acidity.BMC plant biology · 2014Article
- Imaging an optogenetic pH sensor reveals that protons mediate lateral inhibition in the retina.Nature neuroscience · 2014Article
- Article
- Luminal and cytosolic pH feedback on proton pump activity and ATP affinity of V-type ATPase from Arabidopsis.The Journal of biological chemistry · 2012Article
- Vacuolar-type H+-ATPase-mediated proton transport in the rat parietal cell.Pflugers Archiv : European journal of physiology · 2012Article
- Article
- In situ measurement of the electrical potential across the phagosomal membrane using FRET and its contribution to the proton-motive force.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Isolated vacuoles from the yeast Saccharomyces cerevisiae were examined in the whole-vacuole mode of patch recording, to get a detailed functional description of the vacuolar proton pump, the V-ATPase. Functioning of the V-ATPase was characterized by its current-voltage (I-V) relationship, obtained for various levels of vacuolar and cytosolic pH. I-V curves for the V-ATPase were computed as the difference between I-V curves obtained with the pump switched on (ATP, ADP, and Pi present) or off (no ATP). These difference current-voltage relationships usually crossed the voltage axis within the experimental range (from -80 to +80 mV), thus measuring the reversal voltage (ER) for the V-ATPase, which could be compared with the standing ion gradients and free energy of ATP hydrolysis, to calculate the apparent pump stoichiometry or coupling ratio: the number of protons transported for each ATP molecule hydrolyzed. This ratio was found to depend strongly upon the pH difference (DeltapH) across the vacuolar membrane, being approximately 2H+/ATP at high DeltapH (4 pH units) and increasing to >4H+/ATP for small or zero DeltapH. That result is in quantitative agreement with previous determinations on plant vacuoles. Considerations of purely electrical behavior, together with the physical properties of a recent detailed structural model for V-ATPases, led to a linear equivalent circuit--which quantitatively accounts for all observations of variable coupling ratios in fungal and plant V-ATPases by variations of the conductance for bona fide proton pumping (GP) through the ATPase relative to independent proton shunting (GS) through the same protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.