ArticleMicrobial pathogenesis2004
Role of fusion protein cleavage site in the virulence of Newcastle disease virus.
Article in Microbial pathogenesis, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 178 papers, 2 of them syntheses that pooled it.
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Who cites it
178 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Oncolytic Viruses for Cancer Therapy in Dogs.Viruses · 2026Pooled it
- Insights into the multifunctionality of viral glycoproteins F and HN in the lifecycle and pathogenesis of Newcastle disease virus: a systematic review.Veterinary research · 2025Pooled it
- Avian paramyxovirus type 1-associated severe pneumonia in humans: Molecular characterization and zoonotic transmission risk.One health (Amsterdam, Netherlands) · 2026Article
- A divergent betacoronavirus with a functional furin cleavage site in South American bats.Nature communications · 2026Article
- Newcastle Disease Virus Fusion and Haemagglutinin-Neuraminidase Gene Divergence: Implications for Vaccines.Veterinary sciences · 2026Article
- Chicken microRNA 26a-5p regulates replication of Newcastle disease virus by direct targeting of the viral polymerase.Virology journal · 2026Article
- Molecular Characterization of Publicly Available NDV F Gene Sequences From Bangladesh (2010-2024).Veterinary medicine international · 2026Article
- Whole genome sequence analysis and characterization of virulent Newcastle disease virus isolates from chicken and pheasants during 2020/21 outbreaks in Nepal.Virology journal · 2025Article
- Beyond Processing: Furin as a Central Hub in Viral Pathogenesis and Genetic Susceptibility.Biomolecules · 2025Review
- Molecular characterization and genotyping of Newcastle disease viruses using whole-genome sequencing from poultry farms in parts of Amhara Regions, and central Ethiopia.Virology journal · 2025Article
- Long-term surveillance of avian avulavirus in wild birds in China from 2003 to 2020.Virologica Sinica · 2025Article
- Genetic Diversity of Newcastle Disease Virus and Its Implications for Vaccine Development.Veterinary sciences · 2025Review
- Comparative pathology of ducks experimentally inoculated with three different recombinant Newcastle disease viruses.The Journal of veterinary medical science · 2025Article
- Evaluating the impact of avian paramyxovirus type 1 infection in poultry at live bird markets in Nigeria: defining hurdles to sustainable agriculture.BMC veterinary research · 2025Article
- A comprehensive pathological and molecular investigation of viral co-infections in ducks in Egypt.Frontiers in microbiology · 2025Article
- Genetic diversity and phylogenetic characterization of Newcastle Disease Virus in broilers in Iraq based on the F gene analysis.Open veterinary journal · 2025Article
- Article
- Characterizing Host microRNA: Virus Interactions ofViruses · 2024Article
- Enhanced Oncolytic Potential of Engineered Newcastle Disease Virus Lasota Strain through Modification of Its F Protein Cleavage Site.Microorganisms · 2024Article
- Article
118 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Newcastle disease virus (NDV) causes a highly contagious and economically important disease in poultry. Viral determinants of NDV virulence are not completely understood. The amino acid sequence at the protease cleavage site of the fusion (F) protein has been postulated as a major determinant of NDV virulence. In this study, we have examined the role of F protein cleavage site sequence in NDV virulence using reverse genetics technology. The sequence G-R-Q-G-R present at the cleavage site of the F protein of avirulent strain LaSota was mutated to R-R-Q-K-R, which is present in the F cleavage site of neurovirulent strain Beaudette C (BC). The resultant mutated LaSota V.F. virus did not require exogenous protease for infectivity in cell culture, indicating that the F protein was cleaved by intracellular proteases. The virulence of the mutant and parental viruses was evaluated in vivo by intracerebral pathogenicity index (ICPI) and intravenous pathogenicity index (IVPI) tests in chickens. Our results showed that the modification of the F protein cleavage site resulted in a dramatic increase in virulence from an ICPI value of 0.00 for LaSota to a value of 1.12 for LaSota V.F. However, the ICPI value of LaSota V.F. was lower than that of BC, which had a value of 1.58. Interestingly, the IVPI tests showed values of 0.00 for both LaSota and LaSota V.F. viruses, compared to the IVPI value of 1.45 of BC. In vitro characteristics of the viruses were also studied. Our results demonstrate that the efficiency of cleavage of the F protein plays an important role if the NDV is delivered directly into the brains of chicks, but there could be other viral factors that probably affect peripheral replication, viremia, or entry into the central nervous system.
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