ArticleThe EMBO journal1992
An S-phase specific release from a transcriptional block regulates the expression of mouse ribonucleotide reductase R2 subunit.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 52 citations in OpenAlex.
- Iron chelation cancer therapy using hydrophilic block copolymers conjugated with deferoxamine.Cancer science · 2021Article
- DNA repair initiation induces expression of ribonucleotide reductase in human chronic lymphocytic leukemia cells.Leukemia & lymphoma · 2014Article
- Activity of ribonucleotide reductase helps determine how cells repair DNA double strand breaks.DNA repair · 2009Article
- Role of eIF3a in regulating cell cycle progression.Experimental cell research · 2009Article
- Ataxia-telangiectasia mutated kinase regulates ribonucleotide reductase and mitochondrial homeostasis.The Journal of clinical investigation · 2007Article
- Role of eIF3 p170 in controlling synthesis of ribonucleotide reductase M2 and cell growth.Oncogene · 2004Article
- Mouse ribonucleotide reductase R2 protein: a new target for anaphase-promoting complex-Cdh1-mediated proteolysis.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Cell cycle regulation of the tobacco ribonucleotide reductase small subunit gene is mediated by E2F-like elements.The Plant cell · 2000Article
- Molecular characterization of tobacco ribonucleotide reductase RNR1 and RNR2 cDNAs and cell cycle-regulated expression in synchronized plant cells.Plant molecular biology · 1998Article
- E2F-dependent mitogenic stimulation of the splicing of transcripts from an S phase-regulated gene.Nucleic acids research · 1997Article
- Cell cycle, DNA damage and heat shock regulate suc22+ expression in fission yeast.Molecular & general genetics : MGG · 1996Article
- The cell cycle-coupled expression of topoisomerase IIalpha during S phase is regulated by mRNA stability and is disrupted by heat shock or ionizing radiation.Molecular and cellular biology · 1996Article
- Lack of functional retinoblastoma protein mediates increased resistance to antimetabolites in human sarcoma cell lines.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- MAZ-dependent termination between closely spaced human complement genes.The EMBO journal · 1994Article
- Control of transcription arrest in intron 1 of the murine adenosine deaminase gene.Molecular and cellular biology · 1994Article
- Structure and promoter characterization of the gene encoding the large subunit (R1 protein) of mouse ribonucleotide reductase.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- An E2F-binding site mediates cell-cycle regulated repression of mouse B-myb transcription.The EMBO journal · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribonucleotide reductase (RR) activity in mammalian cells is closely linked to DNA synthesis. The RR enzyme is composed of two non-identical subunits, proteins R1 and R2. Both proteins are required for holoenzyme activity, which is regulated by S-phase specific de novo synthesis and breakdown of the R2 subunit. In quiescent cells stimulated to proliferate and in elutriated cell populations enriched in the various cell cycle phases the R2 protein levels are correlated to R2 mRNA levels that are low in G0/G1-phase cells but increase dramatically at the G1/S border. Using an R2 promoter-luciferase reporter gene construct we demonstrate an unexpected early activation of the R2 promoter as cells pass from quiescence to proliferation. However, due to a transcriptional block, this promoter activation only results in very short R2 transcripts until cells enter the S-phase, when full-length R2 transcripts start to appear. The position for the transcriptional block was localized to a nucleotide sequence approximately 87 bp downstream from the first exon/intron boundary by S1 nuclease mapping of R2 transcripts from modified in vitro nuclear run-on experiments. These results identify blocking of transcription as a mechanism to control cell cycle regulated gene expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.