ArticlePlanta2004
Cotton-fiber germin-like protein. II: Immunolocalization, purification, and functional analysis.
Article in Planta, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus ofCurrent issues in molecular biology · 2023Article
- Development of Whole Genome SNP-CAPS Markers and Preliminary QTL Mapping of Fruit Pedicel Traits in Watermelon.Frontiers in plant science · 2022Article
- Potential stress tolerance roles of barley germins and GLPs.Development genes and evolution · 2021Article
- The major-effect quantitative trait locus Fnl7.1 encodes a late embryogenesis abundant protein associated with fruit neck length in cucumber.Plant biotechnology journal · 2020Article
- System Analysis ofBiomolecules · 2019Article
- Versatility of germin-like proteins in their sequences, expressions, and functions.Functional & integrative genomics · 2015Article
- Do Cupins Have a Function Beyond Being Seed Storage Proteins?Frontiers in plant science · 2015Article
- Article
- Identification and analysis of the germin-like gene family in soybean.BMC genomics · 2010Article
- Regulation of two germin-like protein genes during plum fruit development.Journal of experimental botany · 2010Article
- Cu/Zn superoxide dismutases in developing cotton fibers: evidence for an extracellular form.Planta · 2008Article
- Gene expression changes and early events in cotton fibre development.Annals of botany · 2007Review
- Genes expression analyses of sea-island cotton (Gossypium barbadense L.) during fiber development.Plant cell reports · 2007Article
- Germin-like genes are expressed during somatic embryogenesis and early development of conifers.Plant molecular biology · 2006Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cotton (Gossypium hirsutum L.) contains a germin-like protein (GLP), GhGLP1, that shows tissue-specific accumulation in fiber. The fiber GLP is an oligomeric, glycosylated protein with a subunit size of approximately 25.5 kDa. Accumulation of GhGLP1 occurs during the period of fiber elongation [4-14 days post-anthesis (DPA)]. During early phases of fiber development (2-4 DPA), GhGLP1 localizes to cytoplasmic vesicles as shown by confocal immunofluorescent microscopy. In slightly older fibers (7-10 DPA), GhGLP1 localizes to the apoplast. In other plants, germins and GLPs have been reported to have enzymatic activities including oxalate oxidase (OxO), superoxide dismutase, and ADP-glucose pyrophosphatase. Cotton fiber extracts did not contain OxO activity, nor did intact fibers stain for OxO activity. A four-step purification protocol involving ammonium sulfate precipitation of a 1.0 M NaCl extract, ion-exchange chromatography on DEAE-Trisacryl M, lectin-affinity chromatography, and gel filtration chromatography resulted in electrophoretically pure GhGLP1. While 1.0 M NaCl extracts from 10-14 DPA fiber contained superoxide dismutase and phosphodiesterase activities, GhGLP1 could be separated from both enzyme activities by the purification protocol. Although a GLP accumulates in the cotton fiber apoplast during cell elongation, the function of this protein in fiber growth and development remains unknown.
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Registered trials
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