Evidence map›Paper›PMID 14634786›Full record

ArticlePlant cell reports2004

Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice.

A Okuzaki, K Toriyama

Abstract read
PubMed Publisher
In one paragraph

Article in Plant cell reports, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
4.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 78 citations in OpenAlex.

  1. Review
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  3. Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Genome Editing in Agriculture: Technical and Practical Considerations.International journal of molecular sciences · 2019
    Review
  11. Article
  12. Review
  13. Article
  14. Review
  15. Review
  16. Review
  17. The Regulatory Status of Genome-edited Crops.Plant biotechnology journal · 2016
    Review
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

A OkuzakiLaboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, 981-8555 Sendai, Japan.
K Toriyama
Tohoku University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Site-specific mutagenesis in a rice genome was obtained by introducing chimeric RNA/DNA oligonucleotides (COs) by means of particle bombardment. Three COs were designed to target the independent codons for Pro-171, Trp-548 and Ser-627 of the endogenous rice acetolactate synthase (ALS) gene so it would confer resistance to ALS-inhibiting herbicides. Sequencing of the ALS gene of herbicide-resistant plants demonstrated that the ALS sequence was modified in a site-specific fashion. The efficiency of gene conversion mediated by COs was estimated to be 1x10(-4). These results demonstrate that CO-directed gene targeting is feasible in rice.

Indexed as

Acetolactate SynthaseBase SequenceBiolisticsChimeraDNA, PlantDNA, RecombinantDrug ResistanceGene ConversionGene TargetingGenome, PlantHerbicidesMutagenesis, Site-DirectedOligonucleotidesOryzaPlants, Genetically ModifiedRNAAcetolactate SynthaseDNA, PlantDNA, RecombinantHerbicidesOligonucleotidesRNARNA, PlantRNA, recombinant

Identifiers

PMID14634786
OpenAlexW2124345527

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.