ArticleThe Journal of physiology2004
Effects of acute and chronic endurance exercise on mitochondrial uncoupling in human skeletal muscle.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 144 citations in OpenAlex.
- Metabolic and structural changes in lower-limb skeletal muscle following neuromuscular electrical stimulation: a systematic review.PloS one · 2013Pooled it
- Effects of exercise followed by a low-carbohydrate diet on peak fat oxidation and mitochondrial coupling control and efficiency in healthy trained men.The Journal of physiology · 2026Trial
- Mitochondrial coupling efficiency and myofiber type related to blood pressure 22 h after high-intensity exercise in premenopausal women.European journal of applied physiology · 2025Article
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- Migration increases mitochondrial oxidative capacity without increasing reactive oxygen species emission in a songbird.The Journal of experimental biology · 2024Article
- Training-Induced Increase in V·OMetabolites · 2023Article
- Ischaemic preconditioning blunts exercise-induced mitochondrial dysfunction, speeds oxygen uptake kinetics but does not alter severe-intensity exercise capacity.Experimental physiology · 2022Article
- Sensing local energetics to acutely regulate mitophagy in skeletal muscle.Frontiers in cell and developmental biology · 2022Review
- A Review of the Role of Endo/Sarcoplasmic Reticulum-Mitochondria CaInternational journal of environmental research and public health · 2021Review
- Factors determining training-induced changes in V̇OJournal of applied physiology (Bethesda, Md. : 1985) · 2021Article
- βCells · 2021Article
- Skeletal Muscle Adaptations to Exercise Training in Young and Aged Horses.Frontiers in aging · 2021Article
- Article
- Uncoupling mitochondrial uncoupling from alternative substrate utilization: implications for heavy intensity exercise.The Journal of physiology · 2020Article
- Exercise Effects on Mitochondrial Function and Lipid Metabolism during Energy Balance.Medicine and science in sports and exercise · 2020Article
- Effects of Acute Exercise on Mitochondrial Function, Dynamics, and Mitophagy in Rat Cardiac and Skeletal Muscles.International neurourology journal · 2019Article
- Exercise-induced mitophagy in skeletal muscle occurs in the absence of stabilization of Pink1 on mitochondria.Cell cycle (Georgetown, Tex.) · 2019Article
- High-intensity interval training changes mitochondrial respiratory capacity differently in adipose tissue and skeletal muscle.Physiological reports · 2018Article
- Potential Causes of Elevated REE after High-Intensity Exercise.Medicine and science in sports and exercise · 2017Article
- Hormetic Property of Ginseng Steroids on Anti-Oxidant Status against Exercise Challenge in Rat Skeletal Muscle.Antioxidants (Basel, Switzerland) · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondrial proteins such as uncoupling protein 3 (UCP3) and adenine nucleotide translocase (ANT) may mediate back-leakage of protons and serve as uncouplers of oxidative phosphorylation. We hypothesized that UCP3 and ANT increase after prolonged exercise and/or endurance training, resulting in increased uncoupled respiration (UCR). Subjects were investigated with muscle biopsies before and after acute exercise (75 min of cycling at 70% of .VO2peak) or 6 weeks endurance training. Mitochondria were isolated and respiration measured in the absence (UCR or state 4) and presence of ADP (coupled respiration or state 3). Protein expression of UCP3 and ANT was measured with Western blotting. After endurance training, .VO2peak, citrate synthase activity (CS), state 3 respiration and ANT increased by 24, 47, 40 and 95%, respectively (all P < 0.05), whereas UCP3 remained unchanged. When expressed per unit of CS (a marker of mitochondrial volume) UCP3 and UCR decreased by 54% and 18%(P < 0.05). CS increased by 43% after acute exercise and remained elevated after 3 h of recovery (P < 0.05), whereas the other muscle parameters remained unchanged. An intriguing finding was that acute exercise reversibly enhanced the capacity of mitochondria to accumulate Ca2+(P < 0.05) before opening of permeability transition pores. In conclusion, UCP3 protein and UCR decrease after endurance training when related to mitochondrial volume. These changes may prevent excessive basal thermogenesis. Acute exercise enhances mitochondrial resistance to Ca2+ overload but does not influence UCR or protein expression of UCP3 and ANT. The increased Ca2+ resistance may prevent mitochondrial degradation and the mechanism needs to be further explored.
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