ArticleThe EMBO journal2003
Large T antigen on the simian virus 40 origin of replication: a 3D snapshot prior to DNA replication.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 62 citations in OpenAlex.
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- Gamma-retrovirus design for efficacious microRNA-mediated gene knock-down and protein co-expression without compromising titers.NAR molecular medicine · 2025Article
- Large T antigen mediated target gene replication improves site-specific recombination efficiency.Frontiers in bioengineering and biotechnology · 2024Article
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- The ring-shaped hexameric helicases that function at DNA replication forks.Nature structural & molecular biology · 2018Review
- Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158.Scientific reports · 2016Article
- Dynamic look at DNA unwinding by a replicative helicase.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Biochemistry: Molecular hurdles cleared with ease.Nature · 2012Article
- Modeling of the SV40 DNA Replication Machine.Genes · 2012Article
- The amino acid linker between the endonuclease and helicase domains of adeno-associated virus type 5 Rep plays a critical role in DNA-dependent oligomerization.Journal of virology · 2012Article
- Binding to retinoblastoma pocket domain does not alter the inter-domain flexibility of the J domain of SV40 large T antigen.Archives of biochemistry and biophysics · 2012Article
- A wolf in sheep's clothing: SV40 co-opts host genome maintenance proteins to replicate viral DNA.PLoS pathogens · 2012Review
- Structure-based analysis of the interaction between the simian virus 40 T-antigen origin binding domain and single-stranded DNA.Journal of virology · 2011Article
- Conformational rearrangements of SV40 large T antigen during early replication events.Journal of molecular biology · 2010Article
- A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Error analysis in the determination of the electron microscopical contrast transfer function parameters from experimental power Spectra.BMC structural biology · 2009Article
- Genotyping schemes for polyomavirus BK, using gene-specific phylogenetic trees and single nucleotide polymorphism analysis.Journal of virology · 2009Article
- Validation of BKV large T-antigen ATP-binding site as a target for drug discovery.Antiviral research · 2009Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Large T antigen is the replicative helicase of simian virus 40. Its specific binding to the origin of replication and oligomerization into a double hexamer distorts and unwinds dsDNA. In viral replication, T antigen acts as a functional homolog of the eukaryotic minichromosome maintenance factor MCM. T antigen is also an oncoprotein involved in transformation through interaction with p53 and pRb. We obtained the three-dimensional structure of the full-length T antigen double hexamer assembled at its origin of replication by cryoelectron microscopy and single-particle reconstruction techniques. The double hexamer shows different degrees of bending along the DNA axis. The two hexamers are differentiated entities rotated relative to each other. Isolated strands of density, putatively assigned to ssDNA, protrude from the hexamer-hexamer junction mainly at two opposite sites. The structure of the T antigen at the origin of replication can be understood as a snapshot of the dynamic events leading to DNA unwinding. Based on these results a model for the initiation of simian virus 40 DNA replication is proposed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.