ArticleGenes & development2003
Structural and biochemical analyses of DNA and RNA binding by a bifunctional homing endonuclease and group I intron splicing factor.
Article in Genes & development, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 92 citations in OpenAlex.
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- Characterization of homing endonuclease binding and cleavage specificities using yeast surface display SELEX (YSD-SELEX).Nucleic acids research · 2017Article
- The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease.Journal of molecular biology · 2016Article
- The diversity of mtDNA rns introns among strains of Ophiostoma piliferum, Ophiostoma pluriannulatum and related species.SpringerPlus · 2016Article
- Recent advances in developing molecular tools for targeted genome engineering of mammalian cells.BMB reports · 2015Review
- Visualizing phosphodiester-bond hydrolysis by an endonuclease.Nature structural & molecular biology · 2015Article
- Massively parallel determination and modeling of endonuclease substrate specificity.Nucleic acids research · 2014Article
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- Homing endonucleases: from genetic anomalies to programmable genomic clippers.Methods in molecular biology (Clifton, N.J.) · 2014Review
- 5'-Cytosine-phosphoguanine (CpG) methylation impacts the activity of natural and engineered meganucleases.The Journal of biological chemistry · 2012Article
- Expanding LAGLIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space.Nucleic acids research · 2012Article
- Mining endonuclease cleavage determinants in genomic sequence data.The Journal of biological chemistry · 2011Article
- Tapping natural reservoirs of homing endonucleases for targeted gene modification.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Context dependence between subdomains in the DNA binding interface of the I-CreI homing endonuclease.Nucleic acids research · 2011Article
- Learning to live together: mutualism between self-splicing introns and their hosts.BMC biology · 2011Review
- Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.Current gene therapy · 2011Review
- Homing endonucleases: from microbial genetic invaders to reagents for targeted DNA modification.Structure (London, England : 1993) · 2011Review
- Generation of redesigned homing endonucleases comprising DNA-binding domains derived from two different scaffolds.Nucleic acids research · 2010Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
We determined the crystal structure of a bifunctional group I intron splicing factor and homing endonuclease, termed the I-AniI maturase, in complex with its DNA target at 2.6 A resolution. The structure demonstrates the remarkable structural conservation of the beta-sheet DNA-binding motif between highly divergent enzyme subfamilies. DNA recognition by I-AniI was further studied using nucleoside deletion and DMS modification interference analyses. Correlation of these results with the crystal structure provides information on the relative importance of individual nucleotide contacts for DNA recognition. Alignment and modeling of two homologous maturases reveals conserved basic surface residues, distant from the DNA-binding surface, that might be involved in RNA binding. A point mutation that introduces a single negative charge in this region uncouples the maturase and endonuclease functions of the protein, inhibiting RNA binding and splicing while maintaining DNA binding and cleavage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.