ArticleThe American journal of pathology2003
Nephrin promotes cell-cell adhesion through homophilic interactions.
Article in The American journal of pathology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 147 citations in OpenAlex.
- Podocin oligomers regulate the ordering of nephrin chains, providing the molecular basis ofProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Hbs and Rst adhesion molecules provide a regional code that regulates cell elimination during epithelial remodeling.iScience · 2026Article
- Structural and functional insights of the podocyte slit diaphragm complex.Tissue barriers · 2026Review
- Quantifiable and reproducible phenotypic assessment of a constitutive knockout mouse model for congenital nephrotic syndrome of the Finnish type.Scientific reports · 2024Article
- A simple protocol to establish a conditionally immortalized mouse podocyte cell line.Scientific reports · 2024Article
- Selective modulator of nuclear receptor PPARγ with reduced adipogenic potential ameliorates experimental nephrotic syndrome.iScience · 2022Article
- Interactions between Phase-Separated Liquids and Membrane Surfaces.Applied sciences (Basel, Switzerland) · 2021Article
- Evolutionary conservation of intrinsically unstructured regions in slit-diaphragm proteins.PloS one · 2021Article
- Review
- Kirrel3-Mediated Synapse Formation Is Attenuated by Disease-Associated Missense Variants.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Phosphorylation of nephrin induces phase separated domains that move through actomyosin contraction.Molecular biology of the cell · 2019Article
- Loss of slit protein nephrin is associated with reduced antioxidant superoxide dismutase expression in podocytes shed from women with preeclampsia.Physiological reports · 2018Article
- Nephron, Wilms' tumor-1 (WT1), and synaptopodin expression in developing podocytes of mice.Experimental animals · 2017Article
- Inducible Expression of Claudin-1 in Glomerular Podocytes Generates Aberrant Tight Junctions and Proteinuria through Slit Diaphragm Destabilization.Journal of the American Society of Nephrology : JASN · 2017Article
- Notoginsenoside R1 ameliorates podocyte injury in rats with diabetic nephropathy by activating the PI3K/Akt signaling pathway.International journal of molecular medicine · 2016Article
- Nephrin Contributes to Insulin Secretion and Affects Mammalian Target of Rapamycin Signaling Independently of Insulin Receptor.Journal of the American Society of Nephrology : JASN · 2016Article
- Avian Podocytes, Which Lack Nephrin, Use Adherens Junction Proteins at Intercellular Junctions.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2016Article
- Role of Neuropilin-1 in Diabetic Nephropathy.Journal of clinical medicine · 2015Review
- Review series: The cell biology of renal filtration.The Journal of cell biology · 2015Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Nephrin is a type-1 transmembrane protein and a key component of the podocyte slit diaphragm, the ultimate glomerular plasma filter. Genetic and acquired diseases affecting expression or function of nephrin lead to severe proteinuria and distortion or absence of the slit diaphragm. Here, we showed by using a surface plasmon resonance biosensor that soluble recombinant variants of nephrin, containing the extracellular part of the protein, interact with each other in a specific and concentration-dependent manner. This molecular interaction was increased by twofold in the presence of physiological Ca(2+)concentration, indicating that the binding is not dependent on, but rather promoted by Ca(2+). Furthermore, transfected HEK293 cells and an immortalized mouse podocyte cell line overexpressing full-length human nephrin formed cellular aggregates, with cell-cell contacts staining strongly for nephrin. The distance between plasma membranes at the nephrin-containing contact sites was shown by electron microscopy to be 40 to 50 nm, similar to the width of glomerular slit diaphragm. The cell contacts could be dissociated with antibodies reacting with the first two extracellular Ig-like domains of nephrin. Wild-type HEK293 cells were shown to express slit diaphragm components CD2AP, P-cadherin, FAT, and NEPH1. The results show that nephrin molecules exhibit homophilic interactions that could promote cellular contacts through direct nephrin-nephrin interactions, and that the other slit diaphragm components expressed could contribute to that interaction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.