ArticleThe American journal of pathology2003
Liver gene expression profiles of rats treated with clofibric acid: comparison of whole liver and laser capture microdissected liver.
Article in The American journal of pathology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- The influence of cancer tissue sampling on the identification of cancer characteristics.Scientific reports · 2015Article
- A mill based instrument and software system for dissecting slide-mounted tissue that provides digital guidance and documentation.BMC clinical pathology · 2013Article
- Nuclear receptors in the multidrug resistance through the regulation of drug-metabolizing enzymes and drug transporters.Biochemical pharmacology · 2012Review
- ADAM12 and ADAM17 gene expression in laser-capture microdissected and non-microdissected breast tumors.Pathology oncology research : POR · 2011Article
- Gene expression analyses of neurons, astrocytes, and oligodendrocytes isolated by laser capture microdissection from human brain: detrimental effects of laboratory humidity.Journal of neuroscience research · 2009Article
- Analysis of differential gene expression in colorectal cancer and stroma using fluorescence-activated cell sorting purification.British journal of cancer · 2009Article
- Impact of sample acquisition and linear amplification on gene expression profiling of lung adenocarcinoma: laser capture micro-dissection cell-sampling versus bulk tissue-sampling.BMC medical genomics · 2009Article
- Stabilization of RNA during laser capture microdissection by performing experiments under argon atmosphere or using ethanol as a solvent in staining solutions.RNA (New York, N.Y.) · 2008Article
- Biologically relevant effects of mRNA amplification on gene expression profiles.BMC bioinformatics · 2006Article
- Macrodissection versus microdissection of rectal carcinoma: minor influence of stroma cells to tumor cell gene expression profiles.BMC genomics · 2005Article
- Laser capture microdissection and single-cell RT-PCR without RNA purification.Journal of immunological methods · 2005Article
- Highly tissue substructure-specific effects of human papilloma virus in mucosa of HIV-infected patients revealed by laser-dissection microscopy-assisted gene expression profiling.The American journal of pathology · 2004Article
- Digitally guided microdissection aids somatic mutation detection in difficult to dissect tumors.Cancer geneticsArticle
- Performance of Automated Dissection on Formalin-Fixed Paraffin-Embedded Tissue Sections for the 21-Gene Recurrence Score Assay.Technology in cancer research & treatmentArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clofibric acid (CLO) is a peroxisome proliferator (PP) that acts through the peroxisome proliferator activated receptor alpha, leading to hepatocarcinogenesis in rodents. CLO-induced hepatocarcinogenesis is a multi-step process, first transforming normal liver cells into foci. The combination of laser capture microdissection (LCM) and genomics has the potential to provide expression profiles from such small cell clusters, giving an opportunity to understand the process of cancer development in response to PPs. To our knowledge, this is the first evaluation of the impact of the successive steps of LCM procedure on gene expression profiling by comparing profiles from LCM samples to those obtained with non-microdissected liver samples collected after a 1 month CLO treatment in the rat. We showed that hematoxylin and eosin (H&E) staining and laser microdissection itself do not impact on RNA quality. However, the overall process of the LCM procedure affects the RNA quality, resulting in a bias in the gene profiles. Nonetheless, this bias did not prevent accurate determination of a CLO-specific molecular signature. Thus, gene-profiling analysis of microdissected foci, identified by H&E staining may provide insight into the mechanisms underlying non-genotoxic hepatocarcinogenesis in the rat by allowing identification of specific genes that are regulated by CLO in early pre-neoplastic foci.
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