Evidence map›Paper›PMID 14630961›Full record

ArticlePlant physiology2003

Generation and analysis of end sequence database for T-DNA tagging lines in rice.

Suyoung An, Sunhee Park, Dong-Hoon Jeong, Dong-Yeon Lee, Hong-Gyu Kang, Jung-Hwa Yu, Junghe Hur, Sung-Ryul Kim, Young-Hea Kim, Miok Lee and 18 more

Abstract read
In one paragraph

Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.

0numbers the graph read from it
0cells of the map it votes in
99citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

99 citing papers in PubMed.

  1. Article
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  4. Review
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  14. Function Analysis of theInternational journal of molecular sciences · 2022
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  18. Overexpression ofFrontiers in plant science · 2021
    Article
  19. Article
  20. Article

39 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors.

Suyoung AnNational Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
Sunhee Park
Dong-Hoon Jeong
Dong-Yeon Lee
Hong-Gyu Kang
Jung-Hwa Yu
Junghe Hur
Sung-Ryul Kim
Young-Hea Kim
Miok Lee
Soonki Han
Soo-Jin Kim
Jungwon Yang
Eunjoo Kim
Soo Jin Wi
Hoo Sun Chung
Jong-Pil Hong
Vitnary Choe
Hak-Kyung Lee
Jung-Hee Choi
Jongmin Nam
Seong-Ryong Kim
Phun-Bum Park
Ky Young Park
Woo Taek Kim
Sunghwa Choe
Chin-Bum Lee
Gynheung An

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We analyzed 6749 lines tagged by the gene trap vector pGA2707. This resulted in the isolation of 3793 genomic sequences flanking the T-DNA. Among the insertions, 1846 T-DNAs were integrated into genic regions, and 1864 were located in intergenic regions. Frequencies were also higher at the beginning and end of the coding regions and upstream near the ATG start codon. The overall GC content at the insertion sites was close to that measured from the entire rice (Oryza sativa) genome. Functional classification of these 1846 tagged genes showed a distribution similar to that observed for all the genes in the rice chromosomes. This indicates that T-DNA insertion is not biased toward a particular class of genes. There were 764, 327, and 346 T-DNA insertions in chromosomes 1, 4 and 10, respectively. Insertions were not evenly distributed; frequencies were higher at the ends of the chromosomes and lower near the centromere. At certain sites, the frequency was higher than in the surrounding regions. This sequence database will be valuable in identifying knockout mutants for elucidating gene function in rice. This resource is available to the scientific community at http://www.postech.ac.kr/life/pfg/risd.

Indexed as

Base SequenceDNA, BacterialDNA PrimersDNA, Single-StrandedExonsGenetic VectorsIntronsMutagenesis, InsertionalOryzaPlants, Genetically ModifiedPolymerase Chain ReactionSequence Tagged SitesDNA, BacterialDNA PrimersDNA, Single-StrandedT-DNA

Identifiers

PMID14630961
PMCPMC300755

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.