ArticleNucleic acids research2003
Validation of a novel, fully integrated and flexible microarray benchtop facility for gene expression profiling.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
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- Investigating the shared genetic architecture between hypothyroidism and rheumatoid arthritis.Frontiers in immunology · 2023Article
- A comprehensive gene-centric pleiotropic association analysis for 14 psychiatric disorders with GWAS summary statistics.BMC medicine · 2021Article
- Human microRNAs profiling in response to influenza A viruses (subtypes pH1N1, H3N2, and H5N1).Experimental biology and medicine (Maywood, N.J.) · 2016Article
- Comparison of microRNA expression in hippocampus and the marginal division (MrD) of the neostriatum in rats.Journal of biomedical science · 2013Article
- Chromosome 19 annotations with disease speciation: a first report from the Global Research Consortium.Journal of proteome research · 2013Article
- Identification of novel growth phase- and media-dependent small non-coding RNAs in Streptococcus pyogenes M49 using intergenic tiling arrays.BMC genomics · 2012Article
- Temporal- and strain-specific host microRNA molecular signatures associated with swine-origin H1N1 and avian-origin H7N7 influenza A virus infection.Journal of virology · 2012Article
- Transcriptome analysis of early surface-associated growth of Shewanella oneidensis MR-1.PloS one · 2012Article
- Comparative genome-wide analysis of small RNAs of major Gram-positive pathogens: from identification to application.Microbial biotechnology · 2010Review
- Unique genome-wide transcriptome profiles of chicken macrophages exposed to Salmonella-derived endotoxin.BMC genomics · 2010Article
- High-definition DNA methylation profiles from breast and ovarian carcinoma cell lines with differing doxorubicin resistance.PloS one · 2010Article
- Down-regulation of defense genes and resource allocation into infected roots as factors for compatibility between Fagus sylvatica and Phytophthora citricola.Functional & integrative genomics · 2010Article
- ArcS, the cognate sensor kinase in an atypical Arc system of Shewanella oneidensis MR-1.Applied and environmental microbiology · 2010Article
- Deciphering the porcine intestinal microRNA transcriptome.BMC genomics · 2010Article
- Decoding pooled RNAi screens by means of barcode tiling arrays.BMC genomics · 2010Article
- Microarray-based multicycle-enrichment of genomic subsets for targeted next-generation sequencing.Genome research · 2009Article
- Microfluidic Reactor Array Device for Massively Parallel In-situ Synthesis of Oligonucleotides.Sensors and actuators. B, Chemical · 2009Article
- Overview of electrochemical DNA biosensors: new approaches to detect the expression of life.Sensors (Basel, Switzerland) · 2009Article
- Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: comparison of single base mismatches and base bulges.BMC biotechnology · 2008Article
Corrections and comments
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Authors and funding
34 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we describe a novel microarray platform that integrates all functions needed to perform any array-based experiment in a compact instrument on the researcher's laboratory benchtop. Oligonucle otide probes are synthesized in situ via a light- activated process within the channels of a three-dimensional microfluidic reaction carrier. Arrays can be designed and produced within hours according to the user's requirements. They are processed in a fully automatic workflow. We have characterized this new platform with regard to dynamic range, discrimination power, reproducibility and accuracy of biological results. The instrument detects sample RNAs present at a frequency of 1:100 000. Detection is quantitative over more than two orders of magnitude. Experiments on four identical arrays with 6398 features each revealed a mean coefficient of variation (CV) value of 0.09 for the 6398 unprocessed raw intensities indicating high reproducibility. In a more elaborate experiment targeting 1125 yeast genes from an unbiased selection, a mean CV of 0.11 on the fold change level was found. Analyzing the transcriptional response of yeast to osmotic shock, we found that biological data acquired on our platform are in good agreement with data from Affymetrix GeneChips, quantitative real-time PCR and--albeit somewhat less clearly--to data from spotted cDNA arrays obtained from the literature.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.