ArticleNucleic acids research2003
Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 79 citations in OpenAlex.
- Cloning and Functional Characterization of AhyAP-T65Lig, an ATP-Dependent DNA Ligase from Trabzonvirus AP-T65.Molecular biotechnology · 2026Article
- Structure/function studies of the NADExtremophiles : life under extreme conditions · 2023Article
- Bacteriophage origin of some minimal ATP-dependent DNA ligases: a new structure from Burkholderia pseudomallei with striking similarity to Chlorella virus ligase.Scientific reports · 2021Article
- Structural intermediates of a DNA-ligase complex illuminate the role of the catalytic metal ion and mechanism of phosphodiester bond formation.Nucleic acids research · 2019Article
- DNA binding with a minimal scaffold: structure-function analysis of Lig E DNA ligases.Nucleic acids research · 2018Article
- Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe.Bioengineered · 2017Article
- Enzyme-adenylate structure of a bacterial ATP-dependent DNA ligase with a minimized DNA-binding surface.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Hippocampal telomerase is involved in the modulation of depressive behaviors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Protein analysis based on molecular beacon probes and biofunctionalized nanoparticles.Science China. Chemistry · 2010Review
- Microfluidic means of achieving attomolar detection limits with molecular beacon probes.Lab on a chip · 2009Article
- Molecular engineering of DNA: molecular beacons.Angewandte Chemie (International ed. in English) · 2009Review
- Immobilized molecular beacons: a new strategy using UV-activated poly(methyl methacrylate) surfaces to provide large fluorescence sensitivities for reporting on molecular association events.Analytical biochemistry · 2007Article
- Real-time investigation of nucleic acids phosphorylation process using molecular beacons.Nucleic acids research · 2005Article
- Analyzing ligation mixtures using a PCR based method.Biological procedures online · 2005Article
- RNA surveillance: molecular approaches in transcript quality control and their implications in clinical diseases.Molecular medicine (Cambridge, Mass.)Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acids ligation is a vital process in the repair, replication and recombination of nucleic acids. Traditionally, it is assayed by denatured gel electrophoresis and autoradiography, which are not sensitive, and are complex and discontinuous. Here we report a new approach for ligation monitoring using molecular beacon DNA probes. The molecular beacon, designed in such a way that its sequence is complementary with the product of the ligation process, is used to monitor the nucleic acid ligation in a homogeneous solution and in real-time. Our method is fast and simple. We are able to study nucleic acids ligation kinetics conveniently and to determine the activity of DNA ligase accurately. We have studied different factors that influence DNA ligation catalyzed by T4 DNA ligase. The major advantages of our method are its ultrasensitivity, excellent specificity, convenience and real-time monitoring in homogeneous solution. This method will be widely useful for studying nucleic acids ligation process and other nucleic acid interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.