ArticleNucleic acids research2003
The Frog Prince: a reconstructed transposon from Rana pipiens with high transpositional activity in vertebrate cells.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 174 citations in OpenAlex.
- Exploring transposable elements: new horizons in cancer diagnostics and therapeutics.Mobile DNA · 2025Review
- DNA binding and transposition activity of the Sleeping Beauty transposase: role of structural stability of the primary DNA-binding domain.Nucleic acids research · 2025Article
- Ancestral genome reconstruction enhances transposable element annotation by identifying degenerate integrants.Cell genomics · 2024Article
- Functional Characterization of the N-Terminal Disordered Region of theInternational journal of molecular sciences · 2022Article
- Artificial optimization of bambooFrontiers in plant science · 2022Article
- Accelerating and de-risking CMC development with transposon-derived manufacturing cell lines.Biotechnology and bioengineering · 2021Article
- Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications ofInternational journal of molecular sciences · 2021Review
- A native, highly active Tc1/mariner transposon from zebrafish (ZB) offers an efficient genetic manipulation tool for vertebrates.Nucleic acids research · 2021Article
- Intruder (DD38E), a recently evolved sibling family of DD34E/Tc1 transposons in animals.Mobile DNA · 2020Article
- Traveler, a New DD35E Family of Tc1/Mariner Transposons, Invaded Vertebrates Very Recently.Genome biology and evolution · 2020Article
- Evolution ofMobile DNA · 2020Article
- Phylogenetic analysis of theMobile DNA · 2020Article
- Affinities of Terminal Inverted Repeats to DNA Binding Domain of Transposase Affect the Transposition Activity of BambooInternational journal of molecular sciences · 2019Article
- Early heading 7 interacts with DTH8, and regulates flowering time in rice.Plant cell reports · 2019Article
- Article
- Transposons As Tools for Functional Genomics in Vertebrate Models.Trends in genetics : TIG · 2017Review
- DNA Transposition at Work.Chemical reviews · 2016Review
- Tc1-like Transposase Thm3 of Silver Carp (Hypophthalmichthys molitrix) Can Mediate Gene Transposition in the Genome of Blunt Snout Bream (Megalobrama amblycephala).G3 (Bethesda, Md.) · 2015Article
- Sleeping Beauty transposon system for genetic etiological research and gene therapy of cancers.Cancer biology & therapy · 2015Review
- Excision efficiency is not strongly coupled to transgenic rate: cell type-dependent transposition efficiency of sleeping beauty and piggyBac DNA transposons.Human gene therapy methods · 2014Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the Tc1/mariner superfamily of transposable elements isolated from vertebrates are transpositionally inactive due to the accumulation of mutations in their transposase genes. A novel open reading frame-trapping method was used to isolate uninterrupted transposase coding regions from the genome of the frog species Rana pipiens. The isolated clones were approximately 90% identical to a predicted transposase gene sequence from Xenopus laevis, but contained an unpredicted, approximately 180 bp region encoding the N-terminus of the putative transposase. None of these native genes was found to be active. Therefore, a consensus sequence of the transposase gene was derived. This engineered transposase and the transposon inverted repeats together constitute the components of a novel transposon system that we named Frog Prince (FP). FP has only approximately 50% sequence similarity to Sleeping Beauty (SB), and catalyzes efficient cut-and-paste transposition in fish, amphibian and mammalian cell lines. We demonstrate high-efficiency gene trapping in human cells using FP transposition. FP is the most efficient DNA-based transposon from vertebrates described to date, and shows approximately 70% higher activity in zebrafish cells than SB. Frog Prince can greatly extend our possibilities for genetic analyses in vertebrates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.