ArticleNucleic acids research2003
A complex pathway for 3' processing of the yeast U3 snoRNA.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- CPF-CF-terminated snoRNAs shuttle through the cytoplasm via an mRNA guard protein-mediated surveillance mechanism.Nature communications · 2026Article
- Small Nucleolar RNAs (snoRNAs) in Cancer: From Biogenesis to Clinical Potential.OncoTargets and therapy · 2026Review
- Article
- The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity.Nature communications · 2021Article
- Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis.RNA (New York, N.Y.) · 2019Article
- Surveillance-ready transcription: nuclear RNA decay as a default fate.Open biology · 2018Review
- Implication of the box C/D snoRNP assembly factor Rsa1p in U3 snoRNP assembly.Nucleic acids research · 2017Article
- Transcriptome-wide analysis of alternative routes for RNA substrates into the exosome complex.PLoS genetics · 2017Article
- Article
- Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation.Nucleic acids research · 2013Article
- Transfer RNA post-transcriptional processing, turnover, and subcellular dynamics in the yeast Saccharomyces cerevisiae.Genetics · 2013Review
- Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast.The FEBS journal · 2009Article
- Evolutionary diversification of the Sm family of RNA-associated proteins.Molecular biology and evolution · 2008Review
- Identification of genes that function in the biogenesis and localization of small nucleolar RNAs in Saccharomyces cerevisiae.Molecular and cellular biology · 2008Article
- RNA recognition by 3'-to-5' exonucleases: the substrate perspective.Biochimica et biophysica acta · 2008Review
- Requirements for nuclear localization of the Lsm2-8p complex and competition between nuclear and cytoplasmic Lsm complexes.Journal of cell science · 2007Article
- Systematic identification of non-coding RNA 2,2,7-trimethylguanosine cap structures in Caenorhabditis elegans.BMC molecular biology · 2007Article
- A bona fide La protein is required for embryogenesis in Arabidopsis thaliana.Nucleic acids research · 2007Article
- The Lsm2-8 complex determines nuclear localization of the spliceosomal U6 snRNA.Nucleic acids research · 2007Article
- Nuclear pre-mRNA decapping and 5' degradation in yeast require the Lsm2-8p complex.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Mature U3 snoRNA in yeast is generated from the 3'-extended precursors by endonucleolytic cleavage followed by exonucleolytic trimming. These precursors terminate in poly(U) tracts and are normally stabilised by binding of the yeast La homologue, Lhp1p. We report that normal 3' processing of U3 requires the nuclear Lsm proteins. On depletion of any of the five essential proteins, Lsm2-5p or Lsm8p, the normal 3'-extended precursors to the U3 snoRNA were lost. Truncated fragments of both mature and pre-U3 accumulated in the Lsm-depleted strains, consistent with substantial RNA degradation. Pre-U3 species were co-precipitated with TAP-tagged Lsm3p, but the association with spliced pre-U3 was lost in strains lacking Lhp1p. The association of Lhp1p with pre-U3 was also reduced on depletion of Lsm3p or Lsm5p, indicating that binding of Lhp1p and the Lsm proteins is interdependent. In contrast, a tagged Sm-protein detectably co-precipitated spliced pre-U3 species only in strains lacking Lhp1p. We propose that the Lsm2-8p complex functions as a chaperone in conjunction with Lhp1p to stabilise pre-U3 RNA species during 3' processing. The Sm complex may function as a back-up to stabilise 3' ends that are not protected by Lhp1p.
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Registered trials
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