ArticleNucleic acids research2003
Dynamics of the metal-dependent transcription factor complex in vivo at the mouse metallothionein-I promoter.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 24 citations in OpenAlex.
- Regulatory Plasticity of Earthworm wMT-2 Gene Expression.International journal of molecular sciences · 2017Article
- Inhibition of endogenous MTF-1 signaling in zebrafish embryos identifies novel roles for MTF-1 in development.Biochimica et biophysica acta · 2014Article
- The role of small ubiquitin-like modifier-interacting motif in the assembly and regulation of metal-responsive transcription factor 1.The Journal of biological chemistry · 2011Article
- Hypoxia acts through multiple signaling pathways to induce metallothionein transactivation by the metal-responsive transcription factor-1 (MTF-1).Biochemistry and cell biology = Biochimie et biologie cellulaire · 2011Article
- Nrf1 and Nrf2 play distinct roles in activation of antioxidant response element-dependent genes.The Journal of biological chemistry · 2008Article
- Zinc-induced formation of a coactivator complex containing the zinc-sensing transcription factor MTF-1, p300/CBP, and Sp1.Molecular and cellular biology · 2008Article
- The zinc-sensing mechanism of mouse MTF-1 involves linker peptides between the zinc fingers.Molecular and cellular biology · 2006Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The in vivo association of transcription factors with the metallothionein-I promoter was examined using chromatin immunoprecipitation (ChIP) assays. The results demonstrated that c-fos is rapidly recruited along with the metal response element-binding transcription factor-1 (MTF-1) to this promoter in response to zinc or cadmium, and that this recruitment is reversed in the visceral yolk sac by a zinc-deficient diet in vivo, and in cultured cells after lowering the zinc concentration in the medium or during prolonged zinc exposure. In contrast, the interactions of c-jun, USF-1, USF-2 and Sp1 with this promoter are metal-independent. Studies of knockout cells revealed that the recruitment of c-fos to the MT-I promoter requires MTF-1, but that c-fos is not essential for recruitment of MTF-1 and metal-induction of MT-I gene expression. Studies of Hepa cells stably-transfected with reporter genes driven by the MT-I promoter suggested two in vivo binding sites for USF-1 and -2. In contrast, Sp1 was apparently associated with a single binding site (upstream of -153 bp). In addition, maximal recruitment of c-fos by metals required sequences and/or other proteins that interact upstream of -153 bp. In summary, these studies extend our understanding of the complexity and dynamics of the transcription factor complex that forms at the MT-I promoter in vivo in response to metals.
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