ArticlePlanta2004
Introns are key regulatory elements of rice tubulin expression.
Article in Planta, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 86 citations in OpenAlex.
- Engineering non-coding DNA Elements in Rice: an Elegant Approach To fine-tune Agronomical Advantageous Traits.Rice (New York, N.Y.) · 2025Review
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- Development of single nucleotide polymorphisms in key genes of taurine and betaine metabolism in Crassostrea hongkongensis and their association with content-related traits.Research square · 2024Article
- How pollen tubes fight for food: the impact of sucrose carriers and invertases ofFrontiers in plant science · 2023Article
- Understanding the Role ofBiology · 2022Article
- Prospects of Non-Coding Elements in Genomic DNA Based Gene Therapy.Current gene therapy · 2022Review
- Genome-Wide Analysis of Tubulin Gene Family in Cassava and Expression of Family MemberPlants (Basel, Switzerland) · 2021Article
- The novel geneHorticulture research · 2020Article
- Shaping Durum Wheat for the Future: Gene Expression Analyses and Metabolites Profiling Support the Contribution of BCAT Genes to Drought Stress Response.Frontiers in plant science · 2020Article
- Genome-wide association study revealed that the TaGW8 gene was associated with kernel size in Chinese bread wheat.Scientific reports · 2019Article
- Protoplast-Esculin Assay as a New Method to Assay Plant Sucrose Transporters: Characterization of AtSUC6 and AtSUC7 Sucrose Uptake Activity in Arabidopsis Col-0 Ecotype.Frontiers in plant science · 2018Article
- Introns as Gene Regulators: A Brick on the Accelerator.Frontiers in genetics · 2018Article
- Gene coding for an elongation factor is involved in resistance against powdery mildew in common bean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- STP10 encodes a high-affinity monosaccharide transporter and is induced under low-glucose conditions in pollen tubes of Arabidopsis.Journal of experimental botany · 2016Article
- Intron-Mediated Enhancement: A Tool for Heterologous Gene Expression in Plants?Frontiers in plant science · 2016Review
- The promoter and the 5'-untranslated region of rice metallothionein OsMT2b gene are capable of directing high-level gene expression in germinated rice embryos.Plant cell reports · 2014Article
- Comparison of the MpEF1α and CaMV35 promoters for application in Marchantia polymorpha overexpression studies.Transgenic research · 2014Article
- The production of male-sterile wheat plants through split barnase expression is promoted by the insertion of introns and flexible peptide linkers.Transgenic research · 2013Article
- Single nucleotide polymorphisms in the leptin-a gene and associations with growth traits in the orange-spotted grouper (Epinephelus coioides).International journal of molecular sciences · 2013Article
- Multiple functionally divergent and conserved copies of alpha tubulin in bdelloid rotifers.BMC evolutionary biology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genomic clones containing elements that regulate transcription of the three known rice ( Oryza sativa L.) alpha-tubulin isotypes ( Ostua1, Ostua2 and Ostua3) have been isolated. We have used these genomic regions to identify the regulatory elements that contribute to the expression of a marker gene ( gusA) in transient assays performed on rice calli derived from mature embryos. In all cases, we found that the first intron was required to achieve high levels of expression. This is consistent with data already reported for the alpha-tubulin isotype1 and indicates that a common regulatory mechanism is active on all the members of the rice alpha-tubulin gene family. The enhancing effect of the first intron was then tested by constructing illegitimate combinations of alpha-tubulin promoter and intron sequences ( Ostua1pro- Ostua2intro; Ostua1pro- Ostua3intro; Ostua2pro- Ostua3intro; Ostua3pro- Ostua2intro) and then by assaying beta-glucuronidase (GUS) activity in transformed rice calli. All illegitimate combinations expressed GUS at high level, suggesting that rice alpha-tubulin promoters and introns can be exchanged among the different isotypes. This did not occur when the intron of the rice beta-tubulin isotype16, known to enhance transcription of its own gene, was used in place of the alpha-tubulin intron. We have also analysed the effect of abscisic acid (ABA) on GUS expression in rice calli transformed with chimeric tubalpha2pro-intro:: gusA and tubalpha3pro-intro:: gusA constructs. ABA was able to reduce GUS expression only in the presence of the tubalpha2pro-intro sequence. We discuss these data in terms of mechanisms that in rice, as opposed to other plants, may control tubulin isotype-specific expression and the involvement of ABA in the regulation of alpha-tubulin expression.
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