ArticlePLoS biology2003
Partially phosphorylated Pho4 activates transcription of a subset of phosphate-responsive genes.
Article in PLoS biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- The nicotinamide mononucleotide adenylyl transferase (NMNAT/Nma1) modulates phosphate-sensing (PHO) signaling independent of its NAD+ synthesis activity in Saccharomyces cerevisiae.FEMS yeast research · 2026Article
- Optogenetic Control of Phosphate-Responsive Genes Using Single-Component Fusion Proteins inACS synthetic biology · 2024Article
- Optogenetic control of phosphate-responsive genes using single component fusion proteins inbioRxiv : the preprint server for biology · 2024Article
- Article
- The nutrient-responsive CDK Pho85 primes the Sch9 kinase for its activation by TORC1.PLoS genetics · 2023Article
- Perfect adaptation achieved by transport limitations governs the inorganic phosphate response inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Phosphate Restriction Promotes Longevity via Activation of Autophagy and the Multivesicular Body Pathway.Cells · 2021Article
- Functional analysis of aPeerJ · 2021Article
- Optogenetic Control Reveals Differential Promoter Interpretation of Transcription Factor Nuclear Translocation Dynamics.Cell systems · 2020Article
- Independent evolution of transcript abundance and gene regulatory dynamics.Genome research · 2020Article
- Phosphate Homeostasis - A Vital Metabolic Equilibrium Maintained Through the INPHORS Signaling Pathway.Frontiers in microbiology · 2020Review
- Article
- Two Distinct Regulatory Mechanisms of Transcriptional Initiation in Response to Nutrient Signaling.Genetics · 2018Article
- Dual role of starvation signaling in promoting growth and recovery.PLoS biology · 2017Article
- OsHAD1, a Haloacid Dehalogenase-Like APase, Enhances Phosphate Accumulation.Plant physiology · 2017Article
- A systematic genetic screen for genes involved in sensing inorganic phosphate availability in Saccharomyces cerevisiae.PloS one · 2017Article
- Vtc5, a Novel Subunit of the Vacuolar Transporter Chaperone Complex, Regulates Polyphosphate Synthesis and Phosphate Homeostasis in Yeast.The Journal of biological chemistry · 2016Article
- Vesicular Trafficking Systems Impact TORC1-Controlled Transcriptional Programs in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2016Article
- Article
- Inositol pyrophosphates: why so many phosphates?Advances in biological regulation · 2015Review
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Authors and funding
4 authors.
Funding
Abstract
A cell's ability to generate different responses to different levels of stimulus is an important component of an adaptive environmental response. Transcriptional responses are frequently controlled by transcription factors regulated by phosphorylation. We demonstrate that differential phosphorylation of the budding yeast transcription factor Pho4 contributes to differential gene expression. When yeast cells are grown in high-phosphate growth medium, Pho4 is phosphorylated on four critical residues by the cyclin-CDK complex Pho80-Pho85 and is inactivated. When yeast cells are starved for phosphate, Pho4 is dephosphorylated and fully active. In intermediate-phosphate conditions, a form of Pho4 preferentially phosphorylated on one of the four sites accumulates and activates transcription of a subset of phosphate-responsive genes. This Pho4 phosphoform binds differentially to phosphate-responsive promoters and helps to trigger differential gene expression. Our results demonstrate that three transcriptional outputs can be generated by a pathway whose regulation is controlled by one kinase, Pho80-Pho85, and one transcription factor, Pho4. Differential phosphorylation of Pho4 by Pho80-Pho85 produces phosphorylated forms of Pho4 that differ in their ability to activate transcription, contributing to multiple outputs.
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