ArticleAAPS pharmSci2003
High glucose concentration in isotonic media alters caco-2 cell permeability.
Article in AAPS pharmSci, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Conformable Device for Independent Measurements of Mucosal and Vascular Barriers in a Complex In Vitro Intestinal Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- In Vitro Oral Cavity Permeability Assessment to Enable Simulation of Drug Absorption.Pharmaceutics · 2025Article
- Improving the Accuracy of Permeability Data to Gain Predictive Power: Assessing Sources of Variability in Assays Using Cell Monolayers.Membranes · 2024Review
- Using in vitro ADME data for lead compound selection: An emphasis on PAMPA pH 5 permeability and oral bioavailability.Bioorganic & medicinal chemistry · 2022Review
- Food (Matrix) Effects on Bioaccessibility and Intestinal Permeability of Major Olive Antioxidants.Foods (Basel, Switzerland) · 2020Article
- The Impact of Dietary Glycemic Index and Glycemic Load on Postprandial Lipid Kinetics, Dyslipidemia and Cardiovascular Risk.Nutrients · 2020Review
- Bacteria Remediate the Effects of Food Additives on Intestinal Function in anFrontiers in nutrition · 2020Article
- Mechanisms of oral absorption improvement for insoluble drugs by the combination of phospholipid complex and SNEDDS.Drug delivery · 2019Article
- Changes in the Expression of Aquaporin-3 in the Gastrointestinal Tract Affect Drug Absorption.International journal of molecular sciences · 2019Article
- Effect of dietary additives on intestinal permeability in bothDisease models & mechanisms · 2018Article
- Investigation of the influence of high glucose on molecular and genetic responses: anGenes & nutrition · 2018Article
- Drug-transporter mediated interactions between anthelminthic and antiretroviral drugs across the Caco-2 cell monolayers.BMC pharmacology & toxicology · 2017Article
- Therapeutic applications of hydrogels in oral drug delivery.Expert opinion on drug delivery · 2014Review
- The effects of intralaboratory modifications to media composition and cell source on the expression of pharmaceutically relevant transporters and metabolizing genes in the Caco-2 cell line.Journal of pharmaceutical sciences · 2012Article
- Receptor-mediated transcytosis: a mechanism for active extravascular transport of nanoparticles in solid tumors.Journal of controlled release : official journal of the Controlled Release Society · 2012Article
- Differentiating passive from transporter-mediated uptake by PepT1: a comparison and evaluation of four methods.The Journal of surgical research · 2011Article
- Glucose homeostasis across human airway epithelial cell monolayers: role of diffusion, transport and metabolism.Pflugers Archiv : European journal of physiology · 2009Article
- Identification of a cranberry juice product that inhibits enteric CYP3A-mediated first-pass metabolism in humans.Drug metabolism and disposition: the biological fate of chemicals · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Caco-2 cell permeability was evaluated in isotonic media containing high (25 mM) or physiological (5.5 mM) glucose concentrations. Transepithelial electrical resistance (TEER) and membrane fluidity were measured to assess glucose-induced alterations in physical barrier properties. In parallel, distribution of the actin filament (F-actin) and zonula occludens-1 (ZO-1) proteins was assessed by confocal microscopy. Transepithelial fluxes of mannitol, hydrocortisone, digoxin, and glycyl sarcosine (Gly-Sar) that permeate the intestinal mucosa by various pathways were measured to quantify the effect of glucose-induced changes on Caco-2 cell permeability. High glucose decreased maximum TEER of cell monolayers by 47%, whereas membrane fluidity at the hydrophobic core and lipid/polar head interphase was significantly increased. F-actin distribution in high glucose cells appeared more diffuse while ZO-1 was unchanged. Mannitol and hydrocortisone fluxes across Caco-2 cells cultured in high glucose increased by 65% and 24%, respectively. In addition, high glucose decreased the maximum transport capacity (Vmax) of PepT-1. P-glycoprotein activity, however, was unchanged. In conclusion, high extracellular glucose concentration in isotonic media significantly alters physical barrier properties of Caco-2 cell monolayers, which predominantly affects transepithelial transport of solutes permeating the cell barrier by paracellular and transcellular passive diffusion and facilitated transport mediated by the proton-dependent oligopeptide transporter (PepT-1).
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Registered trials
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