ArticlePharmaceutical research2003
Serially passaged human nasal epithelial cell monolayer for in vitro drug transport studies.
Article in Pharmaceutical research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 61 citations in OpenAlex.
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- Impact of Hydrophobic, Hydrophilic, and Mucus-Binding Motifs on the Therapeutic Potential of Ceftazidime Analogs for Pulmonary Administration.Antibiotics (Basel, Switzerland) · 2025Article
- Bioengineering Human Upper Respiratory Mucosa: A Systematic Review of the State of the Art of Cell Culture Techniques.Bioengineering (Basel, Switzerland) · 2024Review
- The Path from Nasal Tissue to Nasal Mucosa on Chip: Part 1-Establishing a Nasal In Vitro Model for Drug Delivery Testing Based on a Novel Cell Line.Pharmaceutics · 2023Article
- Applicability of RPMI 2650 and Calu-3 Cell Models for Evaluation of Nasal Formulations.Pharmaceutics · 2022Article
- Different Culture Conditions Affect Drug Transporter Gene Expression, Ultrastructure, and Permeability of Primary Human Nasal Epithelial Cells.Pharmaceutical research · 2020Article
- Improved In Vitro Model for Intranasal Mucosal Drug Delivery: Primary Olfactory and Respiratory Epithelial Cells Compared with the Permanent Nasal Cell Line RPMI 2650.Pharmaceutics · 2019Article
- Is RPMI 2650 a Suitable In Vitro Nasal Model for Drug Transport Studies?European journal of drug metabolism and pharmacokinetics · 2018Review
- The identification of the TRPM8 channel on primary culture of human nasal epithelial cells and its response to cooling.Medicine · 2017Article
- Puerarin transport across rat nasal epithelial cells and the influence of compatibility with peoniflorin and menthol.Drug design, development and therapy · 2017Article
- Human Airway Primary Epithelial Cells Show Distinct Architectures on Membrane Supports Under Different Culture Conditions.Cell biochemistry and biophysics · 2016Article
- TEER measurement techniques for in vitro barrier model systems.Journal of laboratory automation · 2015Review
- Role of tight junctions in signal transduction: an update.EXCLI journal · 2014Review
- Enhancing effect of borneol and muscone on geniposide transport across the human nasal epithelial cell monolayer.PloS one · 2014Article
- Characteristics and functional relevance of apolipoprotein-A1 and cholesterol binding in mammary gland tissues and epithelial cells.PloS one · 2013Article
- A cell-based computational modeling approach for developing site-directed molecular probes.PLoS computational biology · 2012Article
- Expression of tight junction proteins in epithelium including Ck20-positive M-like cells of human adenoids in vivo and in vitro.Journal of molecular histology · 2008Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo evaluate the feasibility of using a serially passaged culture of human nasal epithelial cell monolayers on a permeable support for in vitro drug transport studies. The optimum conditions for passaged culture as well as the correlation between the transepithelial electrical resistance (TEER) value and drug permeability (Papp) were evaluated.
methodsFresh human nasal epithelial cells were collected from normal inferior turbinates and were subcultured repeatedly in serum-free bronchial epithelial cell growth media (BEGM) in petri dishes. The subcultured cells of each passage were seeded onto permeable supports at 5 x 10(5) cells/cm2 and grown in Dulbecco's modified Eagle medium (DMEM). Morphologic characteristics were observed by scanning electron microscopy (SEM). To verify the formation of tight junctions, actin staining and transmission electron microscopy (TEM) studies were conducted. In the drug transport study, [14C]mannitol and budesonide were selected as the paracellular and the transcellular route markers, respectively.
resultsSerially passaged cells were successfully cultured on a permeable support and showed significantly high TEER values up to passage 4. After 14 days of seeding, SEM showed microvilli, and protrusions of cilia and mucin granules were detected by TEM. The paracellular marker [14C]mannitol showed a nearly constant permeability coefficient (Papp) when the TEER value exceeded 500 omega x cm2 regardless of the passage number. However, as expected, budesonide showed a higher permeability coefficient compared to [14C]mannitol and was less affected by the TEER value.
conclusionsHuman nasal epithelial cell monolayers were successfully subcultured on a permeable support up to passage 4. These cell culture methods may be useful in high-throughput screening of in vitro nasal transport studies of various drugs.
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