ArticlePharmaceutical research2003
Reduced phagocytosis of colloidal carriers using soluble CD47.
Article in Pharmaceutical research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Suppressing or Enhancing Macrophage Engulfment through the Use of CD47 and Related Peptides.Bioconjugate chemistry · 2022Review
- The regulation of CD47-SIRPα signaling axis by microRNAs in combination with conventional cytotoxic drugs together with the help of nano-delivery: a choice for therapy?Molecular biology reports · 2021Review
- Efficient Expression of Soluble Recombinant Protein Fused with Core-Streptavidin in Bacterial Strain with T7 Expression System.Methods and protocols · 2020Article
- The Diverse Range of Possible Cell Membrane Interactions with Substrates: Drug Delivery, Interfaces and Mobility.Molecules (Basel, Switzerland) · 2017Review
- Co-coating of receptor-targeted drug nanocarriers with anti-phagocytic moieties enhances specific tissue uptake versus non-specific phagocytic clearance.Biomaterials · 2017Article
- CD47 on artificial structures.Aging · 2015Article
- Liposome-like Nanostructures for Drug Delivery.Journal of materials chemistry. B · 2013Article
- Surface functionalization of gold nanoparticles with red blood cell membranes.Advanced materials (Deerfield Beach, Fla.) · 2013Article
- Engineering antiphagocytic biomimetic drug carriers.Therapeutic delivery · 2013Review
- 'Marker-of-self' functionalization of nanoscale particles through a top-down cellular membrane coating approach.Nanoscale · 2013Article
- Small molecule-gold nanorod conjugates selectively target and induce macrophage cytotoxicity towards breast cancer cells.Small (Weinheim an der Bergstrasse, Germany) · 2012Article
- Perfluorohexane-loaded macrophages as a novel ultrasound contrast agent: a feasibility study.Molecular imaging and biology · 2008Article
- Nanobiotechnology for the Therapeutic Targeting of Cancer Cells in Blood.Cellular and molecular bioengineeringArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThis study was designed to illustrate the feasibility of using soluble CD47 protein to antagonize phagocytosis of colloidal drug carriers by macrophages.
methodsExpression of CD47-streptavidin (CD47-SA) fusion protein was achieved in B21CodonPlus host cells following IPTG induction. Murine macrophage cell line J774A.1, expressing high levels of SIRPalpha, was selected as the biologic model system for phagocytosis. FITC-labeled perfluorocarbon (PFC) emulsions were used as the colloidal carriers to trigger phagocytosis. Microscopy (inverted light and UV-fluorescence) and flow cytometry were used to qualitatively and quantitatively determine the degree of phagocytosis, respectively.
resultsThe bacterially expressed, purified CD47-SA had neither cytotoxic nor cytostatic effects when incubated with J774A.1 cells up to a concentration of 400 nM for 24 h. Phagocytosis of FITC-labeled PFC emulsions was significantly diminished when macrophages were pretreated with 100 nM CD47-SA for 1 h.
conclusionsWe demonstrated that soluble CD47-SA antagonized phagocytosis of colloidal carriers to a significant degree by interaction with macrophage SIRPalpha.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.