ArticleJournal of anatomy2003
In situ hybridization and immunohistochemistry of versican, aggrecan and link protein, and histochemistry of hyaluronan in the developing mouse limb bud cartilage.
Article in Journal of anatomy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 66 citations in OpenAlex.
- Matrix components in the developing bones and cartilage of embryonic chick skulls: an in situ hybridization study.Anatomical science international · 2026Article
- Three-dimensional (3D) hydrogel serves as a platform to identify potential markers of chondrocyte dedifferentiation by combining RNA sequencing.Bioactive materials · 2021Article
- Hyaluronic acid is required for palatal shelf movement and its interaction with the tongue during palatal shelf elevation.Developmental biology · 2020Article
- Analysis of a limb-specific regulatory element in the promoter of the link protein gene.Biochemical and biophysical research communications · 2019Article
- The glycoproteins EDIL3 and MFGE8 regulate vesicle-mediated eggshell calcification in a new model for avian biomineralization.The Journal of biological chemistry · 2019Article
- An in situ hybridization study of perlecan, DMP1, and MEPE in developing condylar cartilage of the fetal mouse mandible and limb bud cartilage.European journal of histochemistry : EJH · 2015Article
- Inhibition of hyaluronan synthesis alters sulfated glycosaminoglycans deposition during chondrogenic differentiation in ATDC5 cells.Histochemistry and cell biology · 2015Article
- Expression of carbonic anhydrase IX in human fetal joints, ligaments and tendons: a potential marker of mechanical stress in fetal development?Anatomy & cell biology · 2013Article
- The effect of desulfation of chondroitin sulfate on interactions with positively charged growth factors and upregulation of cartilaginous markers in encapsulated MSCs.Biomaterials · 2013Article
- Elastic fiber-mediated enthesis in the human middle ear.Journal of anatomy · 2012Article
- Versican knockdown reduces interzone area during early stages of chick synovial joint development.Anatomical record (Hoboken, N.J. : 2007) · 2012Article
- Altered versican cleavage in ADAMTS5 deficient mice; a novel etiology of myxomatous valve disease.Developmental biology · 2011Article
- Increased hyaluronan synthase-2 mRNA expression and hyaluronan accumulation with choroidal thickening: response during recovery from induced myopia.Investigative ophthalmology & visual science · 2010Article
- Versican G1 domain and V3 isoform overexpression results in increased chondrogenesis in the developing chick limb in ovo.Anatomical record (Hoboken, N.J. : 2007) · 2010Article
- Proteolytic cleavage of versican during limb joint development.Anatomical record (Hoboken, N.J. : 2007) · 2010Article
- Immunolocalization of proteoglycans in Meckel's cartilage of the rat.The open dentistry journal · 2009Article
- Conditional inactivation of Has2 reveals a crucial role for hyaluronan in skeletal growth, patterning, chondrocyte maturation and joint formation in the developing limb.Development (Cambridge, England) · 2009Article
- Aggrecan modulation of growth plate morphogenesis.Developmental biology · 2009Article
- Morphogenetic and regulatory mechanisms during developmental chondrogenesis: new paradigms for cartilage tissue engineering.Tissue engineering. Part B, Reviews · 2009Review
- An in situ hybridization study of Runx2, Osterix, and Sox9 in the anlagen of mouse mandibular condylar cartilage in the early stages of embryogenesis.Journal of anatomy · 2008Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
We investigated the expression pattern of versican, aggrecan, link protein and hyaluronan in the developing limb bud cartilage of the fetal mouse using in situ hybridization and/or immunohistochemistry. Versican mRNA and immunostaining were detected in the mesenchymal cell condensation of the future digital bone at E13. Versican mRNA expression rapidly disappeared from the tibial cartilage, as cartilage formation progressed during E13-15, but the immunostaining was gradually replaced by aggrecan immunostaining from the diaphysis. Immunostaining for both molecules thus had a 'nega-posi' pattern and consequently versican immunostaining was still detected at the epiphyseal end at E15. This result indicated that versican functions as a temporary framework in newly formed cartilage matrix. An aggrecan-positive region within the cartilage invariably had intense hyaluronan staining, whereas a versican-positive region also had affinity for hyaluronan within the cartilage, but not in the mesenchymal cell condensation. Therefore, the presence of versican aggregates was not confirmed in the developing limb bud cartilage. Furthermore, although link protein was more closely related with aggrecan than versican during limb bud cartilage formation, there was a discrepancy between the expression of aggrecan and link protein in tibial cartilage at E15. In particular, only a link protein-positive region was present in the marginal area of the metaphysis and the epiphysis at this stage. This finding may indicate a novel role for link protein.
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