ArticleHistochemistry and cell biology2003
Heparanase mRNA expression during fracture repair in mice.
Article in Histochemistry and cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 42 citations in OpenAlex.
- Lack of endogenous parathyroid hormone delays fracture healing by inhibiting vascular endothelial growth factor‑mediated angiogenesis.International journal of molecular medicine · 2018Article
- [Research progress in osteogenesis and osteogenic mechanism of heparan sulfate].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2017Review
- The cast of clasts: catabolism and vascular invasion during bone growth, repair, and disease by osteoclasts, chondroclasts, and septoclasts.Connective tissue research · 2016Review
- Molecular imaging of expression of vascular endothelial growth factor a (VEGF a) in femoral bone grafts transplanted into living mice.Cell transplantation · 2014Article
- Increased expression of heparanase in osteogenic differentiation of rat marrow stromal cells.Experimental and therapeutic medicine · 2013Article
- Heparanase localization during palatogenesis in mice.BioMed research international · 2013Article
- Production of VEGF receptor 1 and 2 mRNA and protein during endochondral bone repair is differential and healing phase specific.Journal of applied physiology (Bethesda, Md. : 1985) · 2010Article
- Heparanase enhances local and systemic osteolysis in multiple myeloma by upregulating the expression and secretion of RANKL.Cancer research · 2010Article
- Bone grafts in craniofacial surgery.Craniomaxillofacial trauma & reconstruction · 2009Article
- Expression profile of genes related to osteoclastogenesis in mouse growth plate and articular cartilage.Histochemistry and cell biology · 2006Article
- News and views in Histochemistry and Cell Biology.Histochemistry and cell biology · 2004Review
- Innovative techniques and applications in histochemistry and cell biology.Histochemistry and cell biology · 2003Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone fracture healing takes place through endochondral ossification where cartilaginous callus is replaced by bony callus. Vascular endothelial growth factor (VEGF) is a requisite for endochondral ossification, where blood vessel invasion of cartilaginous callus is crucial. Heparanase is an endoglucuronidase that degrades heparan sulfate proteoglycans (HSPG) and releases heparin-binding growth factors including VEGF as an active form. To investigate the role of heparanase in VEGF recruitment during fracture healing, the expression of heparanase mRNA and VEGF, and vessel formation were examined in mouse fractured bone. On days 5 and 7 after the fracture, when mesenchymal cells proliferated and differentiated into chondrocytes, heparanase mRNA was detected in osteo(chondro)clasts and their precursors, but not in the inflammatory phase (day 3). On day 10, both VEGF and HSPG were produced by hypertrophic chondrocytes of the cartilaginous callus and by osteoblasts of the bony callus; numerous osteo(chondro)clasts resorbing the cartilage expressed strong heparanase signals. Adjacent to the cartilage resorption sites, angiogenesis with CD31-positive endothelial cells and osteogenesis with osteonectin-positive osteoblasts were observed. On days 14 and 21, osteoclasts in the woven bone tissue expressed heparanase mRNA. These data suggest that by producing heparanase osteo(chondro)clasts contribute to the recruitment of the active form of VEGF. Thus osteo(chondro)clasts may promote local angiogenesis as well as callus resorption in endochondral ossification during fracture healing.
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Registered trials
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