ArticleMolecular biology of the cell2004
Global phenotypic analysis and transcriptional profiling defines the weak acid stress response regulon in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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66 citing papers in PubMed, 159 citations in OpenAlex.
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- Methods for studying microbial acid stress responses: from molecules to populations.FEMS microbiology reviews · 2024Review
- General mechanisms of weak acid-tolerance and current strategies for the development of tolerant yeasts.World journal of microbiology & biotechnology · 2023Review
- Single nucleotide polymorphisms associated with wine fermentation and adaptation to nitrogen limitation in wild and domesticated yeast strains.Biological research · 2023Article
- Adaptive responses of yeast strains tolerant to acidic pH, acetate, and supraoptimal temperature.Applied microbiology and biotechnology · 2023Article
- Global analysis of the yeast knockout phenome.Science advances · 2023Article
- Saccharomyces cerevisiae employs complex regulation strategies to tolerate low pH stress during ethanol production.Microbial cell factories · 2022Article
- QTL mapping of a Brazilian bioethanol strain links the cell wall protein-encoding gene GAS1 to low pH tolerance in S. cerevisiae.Biotechnology for biofuels · 2021Article
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- Screening and Genetic Network Analysis of Genes Involved in Freezing and Thawing Resistance inGenes · 2021Article
- Discovery and identification of medium-chain fatty acid responsive promoters inEngineering in life sciences · 2020Article
- The role of Lactobacillus species in the control of Candida via biotrophic interactions.Microbial cell (Graz, Austria) · 2019Review
- Transcriptomic and chemogenomic analyses unveil the essential role of Com2-regulon in response and tolerance ofMicrobial cell (Graz, Austria) · 2019Article
- Redox control of yeast Sir2 activity is involved in acetic acid resistance and longevity.Redox biology · 2019Article
- Deletion ofBiotechnology for biofuels · 2019Article
- Inactivation of the transcription factor mig1 (YGL035C) in Saccharomyces cerevisiae improves tolerance towards monocarboxylic weak acids: acetic, formic and levulinic acid.Journal of industrial microbiology & biotechnology · 2018Article
- Pdr18 is involved in yeast response to acetic acid stress counteracting the decrease of plasma membrane ergosterol content and order.Scientific reports · 2018Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Weak organic acids such as sorbate are potent fungistatic agents used in food preservation, but their intracellular targets are poorly understood. We thus searched for potential target genes and signaling components in the yeast genome using contemporary genome-wide functional assays as well as DNA microarray profiling. Phenotypic screening of the EUROSCARF collection revealed the existence of numerous sorbate-sensitive strains. Sorbate hypersensitivity was detected in mutants of the shikimate biosynthesis pathway, strains lacking the PDR12 efflux pump or WAR1, a transcription factor mediating stress induction of PDR12. Using DNA microarrays, we also analyzed the genome-wide response to acute sorbate stress, allowing for the identification of more than 100 genes rapidly induced by weak acid stress. Moreover, a novel War1p- and Msn2p/4p-independent regulon that includes HSP30 was identified. Although induction of the majority of sorbate-induced genes required Msn2p/4p, weak acid tolerance was unaffected by a lack of Msn2p/4p. Ectopic expression of PDR12 from the GAL1-10 promoter fully restored sorbate resistance in a strain lacking War1p, demonstrating that PDR12 is the major target of War1p under sorbic acid stress. Interestingly, comparison of microarray data with results from the phenotypic screening revealed that PDR12 remained as the only gene, which is both stress inducible and required for weak acid resistance. Our results suggest that combining functional assays with transcriptome profiling allows for the identification of key components in large datasets such as those generated by global microarray analysis.
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